Pharmacokinetic analysis of intraarticular injection of insulin and its effect on IGF-1 expression in synovial fluid of healthy horses

Autores/as

  • Fernando García-Lacy Universidad Nacional Autónoma de México (UNAM). Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Ciudad de México, México. https://orcid.org/0000-0003-3787-736X
  • Lilia Gutiérrez-Olvera UNAM. FMVZ, Departamento de Fisiología y Farmacología, Ciudad de México, México.
  • María Bernad UNAM. Facultad de Química, Departamento de Farmacia, Ciudad de México, México.
  • Lisa Fortier Cornell University. Ithaca, USA.
  • Francisco Trigo-Tavera UNAM. FMVZ, Departamento de Patología, Ciudad de México, México.
  • Margarita Gómez-Chavarín UNAM. Depto. Fisiología, Facultad de Medicina, Ciudad de México, México.
  • Alejandro Rodríguez-Monterde UNAM. FMVZ, Secretario de Medicina, Ciudad de México, México.

DOI:

https://doi.org/10.22319/rmcp.v13i2.5942

Palabras clave:

Insulin, Intraarticular, Horse, IGF-1, HPLC

Resumen

Insulin induces mitosis on equine chondrocytes in vitro and enhances production of type II collagen. Insulin, when administered intra-articularly, changes the composition of synovial fluid, including the concentrations of glucose, insulin and glycemia levels. The concentration of insulin in the joint was measured by using high-performance liquid chromatography (HPLC), which provided pharmacokinetic values. Six mixed breed horses were administered three different doses of insulin into one antebrachiocarpal joint (10, 15 and 20 IU) and isotonic saline was administered into the contralateral joint. The blood glucose concentration significantly changed through time for all three doses (P<0.0001). No significant differences in protein concentration and cell count in synovial fluid were found between treated and control joints (P>0.05), no significant difference in synovial glucose concentrations was found between treated and control joints (P>0.05). Values obtained by HPLC analyzed with PkAnalyst program revealed that the pharmacokinetic values were dose dependent, there was no significant difference in concentration of blood glucose between the three different doses (P>0.05). ELISA for IGF-1 (insulin-like growth factor 1) revealed a significant difference between treated and control joints (P<0.001). Insulin used in this study proved to be innocuous to the equine joint, no more than 20 IU for a 350-400 kg horse should be administered.

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Biografía del autor/a

Fernando García-Lacy, Universidad Nacional Autónoma de México (UNAM). Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Ciudad de México, México.

Estudiante del programa: Doctorado en Ciencias de la Producción y de la Salud Animal, FMVZ-UNAM.

Lilia Gutiérrez-Olvera, UNAM. FMVZ, Departamento de Fisiología y Farmacología, Ciudad de México, México.

Departamento de Fisiología y Farmacología, FMVZ-UNAM.

María Bernad, UNAM. Facultad de Química, Departamento de Farmacia, Ciudad de México, México.

Departamento de Farmacia, Facultad de Química, UNAM.

Lisa Fortier, Cornell University. Ithaca, USA.

PhD. DACVS. Cornell University, Ithaca, EUA

Francisco Trigo-Tavera, UNAM. FMVZ, Departamento de Patología, Ciudad de México, México.

PhD. MSc. FMVZ-UNAM. Director de CRAI, UNAM

Alejandro Rodríguez-Monterde, UNAM. FMVZ, Secretario de Medicina, Ciudad de México, México.

Jefe del Departamento de Medicina, FMVZ-UNAM

Citas

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Publicado

16.05.2022

Cómo citar

García-Lacy, F., Gutiérrez-Olvera, L., Bernad, M., Fortier, L., Trigo-Tavera, F., Gómez-Chavarín, M., & Rodríguez-Monterde, A. (2022). Pharmacokinetic analysis of intraarticular injection of insulin and its effect on IGF-1 expression in synovial fluid of healthy horses. Revista Mexicana De Ciencias Pecuarias, 13(2), 391–407. https://doi.org/10.22319/rmcp.v13i2.5942
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