Cases frequency and molecular identification of Cryptosporidium spp. in lactating lambs kept grazing in the State of Veracruz, Mexico
DOI:
https://doi.org/10.22319/rmcp.v9i3.4364Keywords:
Cryptosporidium parvum, Lambs, Frequency, Genotyping.Abstract
The objective was to determine the frequency of Cryptosporidium spp., and to identify the species or genotype of the oocysts found, in suckling lambs kept grazing in the Huasteca Alta region, State of Veracruz, Mexico. Fecal samples were collected from 210 lambs Pelibuey, Black Belly, and Katahdin, 7 to 21 d old from 21 sheep farms. Samples were stained with Kinyoun, nested PCR was used to amplify the 18S rRNA gene region of the parasite (830 bp), and positive samples were sequenced. The frequency of animals positive to Cryptosporidium spp by microscopy was 19.5 % (41/210), with a 10 to 50 % range among herds; with molecular techniques, the frequency of positive lambs was 26.8 % (11/41) with a 14 to 50 % range; all other samples were negative to both tests. All 11 samples sequenced showed 100 % homology with the 18S rRNA region of C. parvum. Results confirm the relevance of C. parvum as a major etiology of cryptosporidiosis in lactating lambs and show its broad distribution in this region of Mexico. This identification highlights that sheep can be considered as a significant potential source of human cryptosporidiosis, since it is considered a zoonotic disease, mainly for the people handling the flocks.
Downloads
References
Karanis P, Kourenti C, Smith H. Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J Water Health 2007;5:1-38.
Fayer R, Santín M, Macarisin D. Cryptosporidium ubiquitum n. sp., in animals and humans. Vet Parasitol 2010;172:23–32.
Razzolinia M, Souza De L.M, Hachichc EM, Zanoli MI, Nardoccia AC. Giardia and Cryptosporidium infection risk by simultaneous exposure to drinking water. Microbl Risk Anal 2016;4:1–6.
Quílez J, Torres E, Chalmers RM, Hadfield SJ, Del Cacho E, Sánchez-Acedo C. Cryptosporidium genotypes and subtypes in lambs and goat kids in Spain. Appl Environ Microbiol 2008;74:6026–6031.
Romero-Salas D, Alvarado EC, Cruz RA, Aguilar DM, Ibarra PN, Merino CJ, Pérez LA, Hernández TJ. Prevalence of Cryptosporidium in small ruminants from Veracruz, Mexico. BMC Vet Res 2016;12:14.
Castro–Hermida JAM, González W, Mezo M. Natural infection by Cryptosporidium parvum and Giardia duodenalis in sheep and goats in Galicia (New Spain). Small Ruminant Res 2007;72:96-100.
Santın M, Trout JM, Xiao L, Zhou L, Greiner E, Fayer R. Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet Parasitol 2004;122:103–117.
Fayer R, Santín M. Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries). Vet Parasitol 2009;164:192–200.
Xiao L, Ryan UM. Cryptosporidiosis: an update in molecular epidemiology. Curr Opin Infec Dis 2004;17:483-490.
Khalil S, Mirdhaa BR, Paulb J, Pandaa A, Makhariac G, Chaudhrya R, Bhatnagard S. Development and evaluation of molecular methods for detection of Cryptosporidium spp. in human clinical samples. Exp Parasitol 2016;170:207–213.
Santin M, Trout JM, Fayer R. Prevalence and molecular characterization of Cryptosporidium and Giardia species and genotypes in sheep in Maryland. Vet Parasitol 2007;146:17–24.
Zucatto AS, Aquino MCC, Inácio SV, Figueiredo RN, Pierucci JC, Perri SHV, Meireles MV, Bresciani KDS. Molecular characterization of Cryptosporidium spp. in lambs in the South Central region of the State of São Paulo. Arq Bras Med Vet Zoot Belo Horizonte 2015;67:1678-7067.
Wang Y, Feng Y, Cui B, Jian F, Ning C, Wang R, Zhang L, Xiao L. Cervine genotype is the major Cryptosporidium genotype in sheep in China. Parasitol Res 2010;106:341–347.
Goma FY, Geurden V, Siwila J, Phiri IG, Gabriel S, Claerebout E, Verccruyse J. The prevalence and molecular characterization of Cryptosporidium spp. in small ruminants in Zambia. Small Ruminant Res 2007;72:77–80.
Alonso-Fresán MU, García-Alvarez A, Salazar-García F, Vazquez-Chagoyan JC, Pescador–Salas N, Saltijeral-Oaxaca J. Prevalence of Cryptosporidium spp. in asymptomatic sheep in family flocks from México State. J Vet Med Series B 2005;52:482-483.
García E. Modificación al sistema de clasificación climática de Koeppen. 2a ed. Instituto de Geografía. Universidad Nacional Autónoma de México. 1973.
SAGARPA. Anuario Estadístico y Geográfico del estado de Veracruz http://www.sagarpa.gob.mx, Consultado 12 May, 2012.
Thusfield M. Veterinary epidemiology. 2nd ed. London: Blackwell Science; 1995.
Fayer R, Trout JM, Craczyk TD, Lewis EJ. Prevalence of Cryptosporidium, Giardia, and Eimeria infections in post-weaned and adult cattle on three Maryland farms. Vet Parasitol 2000;93:103–112.
Baxby D, Blundell N, Hart CA. The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces. J Hyg (Lond) 1984;93:317-23.
Xiao L, Morgan U, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RCA, Fayer R, Lal A. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. App Environ Microbiol 1999;65:3386–3391.
Stephen AF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389-3402.
Al-Habsi K, Yang R, Williams A, Miller D, Ryan U, Jacobson C. Zoonotic Cryptosporidium and Giardia shedding by captured rangeland goats. Vet Parasitol: Region Stud Rep 2017;7:32–35
Geurden T, Thomas P, Casaert S, Vercruysse J, Claerebout E. Prevalence and molecular characterization of Cryptosporidium and Giardia in lambs and goat kids in Belgium. Vet Parasitol 2008;155:142-145.
Sari B, Arslan MO, Gicik Y, Kara M, Tasci GT. The prevalence of Cryptosporidium species in diarrheic lambs in Kars province and potential risk factors. Trop Anim Health Prod 2009;41:819-826.
Ahamed, I, Yadav, A, Katoch, R, Godara, R, Saleem, Taniya, Nisar, NA. Prevalence and analysis of associated risk factors for Cryptosporidium infection in lambs in Jammu district. J Parasit Dis 2015;39:414–417.
Causapé AC, Quilez J, Sanchez-Acedo C, del Cacho E, López-Bernad F. Prevalence and analysis of potential risk factors for Cryptosporidium parvum infection in lambs in Zaragoza (northeastern Spain). Vet Parasitol 2002;104:287-298.
Xiao L, Fayer R. Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int J Parasitol 2008;38:1239-1255.
Kim CW. Laboratory animal models for experimental cryptosporidiosis: a mini-review. Res Rev Parasitol 1994;54:13-28.
Vergara C, Quiles J. Criptosporidiosis: una zoonosis parasitaria. Rev MVZ Córdoba 2004;9:363-372.
Taylan-Ozkana A, Yasa-Durub S, Uslucac S, Lysend C, Yed J, Roelligd DM, Fenge Y, Xiao L. Cryptosporidium species and Cryptosporidium parvum subtypes in dairy calves and goat kids reared under traditional farming systems in Turkey. Exp Parasitol 2016;170:16–20.
Mueller-Doblies D, Giles M, Elwin K, Smith RP, Clifton-Hadley FA, Chalmers RM. Distribution of Cryptosporidium species in sheep in the UK. Vet Parasitol 2008;154:214–219.
Castillo-García C, Cruz-Vázquez C, López RR, Sánchez GM, Rosario CR, Vitela MI, Medina EL. Frequency and molecular identification of Cryptosporidium spp in confined suckling dairy calves in Aguascalientes, Mexico. Téc Pecu Méx 2009;47:425-434.
Paoletti B, Giangaspero A, Gatti A, Iorio R, Cembalo D, Milillo P, Traversa D. Immunoenzymatic analysis and genetic detection of Cryptosporidium parvum in lambs from Italy. Exp Parasitol 2009;122:349-352.
Downloads
Published
How to Cite
-
Abstract1201
-
PDF (Español)678
-
XML (Español)503
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
