Molecular prediction of serotypes of Streptococcus suis isolated from pig’s farms in Mexico
DOI:
https://doi.org/10.22319/rmcp.v12i3.5668Palabras clave:
gdh PCR, Pig farms, Serotypes, Streptococcus suisResumen
Infections caused by Streptococcus suis (S. suis) pose a problem for the pig industry worldwide. Pigs often carry multiple serotypes of S. suis in the upper respiratory tract, where S. suis is frequently isolated from. The clinical diagnosis of the infection is presumptive and is generally based on clinical signs, the age of the animal and macroscopic lesions. In the laboratory, identification of S. suis is performed biochemically, and then, serotyping is performed with antisera to determine the serotype, but these tests can be inconclusive. To date, there are few studies that have documented the presence and diversity of S. suis serotypes in Mexico. In the present study, it was characterized S. suis strains from Mexican pig farms using molecular approaches; samples were first processed by PCR of the gdh gene to detect S. suis. Positive samples were then subjected to a two-step multiplex PCR (cps PCR) to detect and characterize each strain; the first step consisted of a grouping PCR and the second step consisted of a typing PCR. The serotypes detected in the pig farming areas of Mexico included 1/2, 2, 3, 5, 7, 8, 9, 17, and 23. These findings are important for the characterization of serotypes present in Mexico and for outbreak prevention.
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Goyette-Desjardins G, Auger JP, Xu J, Segura M, Gottschalk M. Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing. EMI 2014;3:45. doi:10.1038/emi.2014.45.
Flores JL, Higgins R, D'Allaire S, Charette R, Boudreau M, Gottschalk M. Distribution of the different capsular types of Streptococcus suis in nineteen swine nurseries. Can Vet J 1993;34(3):170–171.
Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Gregory WS, Zhang J. Diseases of swine. 11th ed. Streptococcosis. John Wiley & Sons, Inc; 2019.
Wisselink HJ, Smith HE, Stockhofe-Zurwieden N, Peperkamp K, Vecht U. Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet Microbiol 2000;74:237–248. doi:10.1016/s0378-1135(00)00188-7.
Liu Z, Zheng H, Gottschalk M, Bai X, Lan R, Ji S, Xu J. Development of multiplex PCR assays for the identification of the 33 serotypes of Streptococcus suis. PLoS One 2013;8(8):e72070. doi:10.1371/journal.pone.0072070.
Haas B, Grenier D. Understanding the virulence of Streptococcus suis: A veterinary, medical, and economic challenge. Méd Mal Infect 2018;48(3):159–166. doi:10.1016/j.medmal.2017.10.001.
Gottschalk M. Porcine Streptococcus suis strains as potential sources of infections in humans: An underdiagnosed problem in North America? JSAP 2004;12(4):197–199.
Okura M, Lachance C, Osaki M, Sekizaki T, Maruyama F, Nozawa T, Takamatsu D. Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis. J Clin Microbiol 2014;52:1714–1719. doi:10.1128/jcm.03411-13.
Okwumabua O, O'Connor M, Shull E. A polymerase chain reaction (PCR) assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase. FEMS Microbiology Letters 2003;218(1):79–84. doi:10.1111/j.1574-6968.2003.tb11501.
Gottschalk M, Lacouture S, Bonifait L, Roy D, Fittipaldi N, Grenier D. Characterization of Streptococcus suis isolates recovered between 2008 and 2011 from diseased pigs in Quebec, Canada. Vet Microbiol 2013;162:819–825. doi:10.1016/j.vetmic.2012.10.028.
Kerdsin A, Akeda Y, Hatrongjit R, Detchawna U, Sekizaki T, Hamada S, Oishi K. Streptococcus suis serotyping by a new multiplex PCR. J Med Microbiol 2014;63:824–830. doi:10.1099/jmm.0.069757-0.
Okura M, Osaki M, Nomoto R, Arai S, Osawa R, Sekizaki T, Takamatsu D. Current Taxonomical situation of Streptococcus suis. Pathogens 2016;5(3):45.
Straw BE, Zimmerman, JJ, D´Allaire S, Taylor DJ. Diseases of swine. 9th ed. Streptococcosis. Blackwell Publishing. 2006.
Zheng H, Du P, Qiu X, Kerdsin A, Roy D, Bai X, Xu J, Vela AI, Gottschalk M. Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada. Vet Res 2019;49(1):1. doi: 10.1186/s13567-017-0498-2. Erratum in: Vet Res. 2019;16;50(1):62. PMID: 29316972; PMCID: PMC5759227.
Wei Z, Li R, Zhang A, He H, Hua Y, Xia J, Jin M. Characterization of Streptococcus suis isolates from the diseased pigs in China between 2003 and 2007. Vet Microbiol 2009;137(1-2):196–201. doi:10.1016/j.vetmic.2008.12.015.
Gottschalk M, Lacouture S. Canada: Distribution of Streptococcus suis (from 2012 to 2014) and Actinobacillus pleuropneumoniae (from 2011 to 2014) serotypes isolated from diseased pigs. Can Vet J 2015;56(10):1093-1094.
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