Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico
DOI:
https://doi.org/10.22319/rmcp.v12i1.5378Keywords:
Bacteriophages, Escherichia coli, Genes, ResistanceAbstract
Antibiotic resistance genes (ARG) have been described mainly in bacteria, but are known to occur in temperate phages. Prevalence of the qnrB, qnrA and blaTEM genes was identified in Escherichia coli strains and temperate phages by lytic cycle induction. From a total of 48 samples collected from drinking water, wastewater and sewer water in slaughterhouses in the State of Mexico, Mexico, 37 contained E. coli isolates. Resistance was highest to tetracycline (32/37; 86.4 %), followed by trimethoprim-sulfamethoxazole (19/37; 51.3 %) and ampicillin and nalidixic acid (18/37; 48.6 %). Prevalence of the blaTEM gene was 37.8 % in the bacterial isolates and 3.5 % in the phage isolates. The bacterial isolates contained 8.1 % qnrA and 29.7 % qnrB genes, while the phage isolates contained 2.7 and 24.3 %, respectively. Presence of ARG in the bacterial isolates was linked to phage DNA, highlighting the significant role it plays in the spread of ARG in the studied slaughterhouses. Understanding the mechanisms of antimicrobial resistance will contribute to developing effective control measures.
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References
Balcazar JL. Bacteriophages as vehicles for antibiotic resistance genes in the environment. PLoS Pathog 2014;10(7):e1004219.
Colomer-Lluch M, Jofre J, Muniesa M. Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples. PLoS One 2011;6(3):e17549 .
Colomer-Lluch M, Calero-Cáceres W, Jebri S, Hmaied F, Muniesa M, Jofre, J. Antibiotic resistance genes in bacterial and bateriophage fractions of Tunisian and Spanish wastewater as markers to compare the antibiotic resistance patterns in each population. Environment Int 2014a;(73):167-175.
Quirós P, Colomer-Lluch M, Martínez-Castillo A, Miró E, Argente M, Jofre J, et al. Antibiotic resistance genes in the bacteriophage DNA fraction of human fecal samples. Antimicrob Agents Chemother 2014;58(1):606-609.
Iversen H, L´Ábée-Lund TM, Aspholm M, Arnesen LP, Lindbäck T. Commensal E. coli Stx2 lysogens produce high levels of phages after spontaneous propahge induction. Front Cell Infect Microbiol 2015;(5):1-8.
Jaramillo ACJ, Martínez MJJ. Epidemiología Veterinaria. 1ra ed. México: El Manual Moderno; 2010.
SS. Secretaria de Salud. Salud Ambiental. Agua para uso y consumo humano, requisitos sanitarios que se deben cumplir en los sistemas de abastecimiento públicos y privados durante el manejo del agua. Procedimientos sanitarios para el muestreo. NOM-230-SSA1-2002. Diario Oficial de la Federación, México.
Aguilar MOS, Talavera RM, Soriano VE, Barba LJ, Vazquez, NJ. Determination of extended spectrum -lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico. Trop Anim Health Prod 2015;47(5):975-981.
CLSI. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial disk susceptibility tests, 19th ed. Approved Standard M02-A11. Wayne, PA: CLSI. 2012.
CLSI. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial susceptibility testing, 25nd informational supplement. M100-S25. Wayne, PA: CLSI. 2015.
Raya RR, Hébert EM. Isolation of phage via induction of lysogens. In: Clokie MRJ, Kropinski AM, editors. Bacteriophages. Methods and Protocols, Volume 1: Isolation, characterization, and interactions. UK: Humana Press; 2009:23-32.
Pickard DJ. Preparation of bacteriophage lysate and pure DNA. In: Clokie MRJ, Kropinski AM, editors. Bacteriophages. Methods and protocols, Volume 2: Molecular and Applied Aspects. UK: Humana Press; 2009:3-9.
Ahmed AM, Ishida Y, Shimamoto T. Molecular characterization of antimicrobial resistance in Salmonella isolated from animals in Japan. J Appl Microbiol 2009;106(2):402-409.
Kraychete GB, Botelho LA, Campana EH, Picão RC, Bonelli RR. Updated multiplex PCR for detection of all six plasmid-mediated qnr gene families. Antimicrob Agents Chemother 2016;60(12):7524-7526.
Dallenne C, Da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother 2010;65(3):490-495.
Ouedraogo SA, Sanou M, Kissou A, Sanou S, Solaré H, Kaboré F, et al. High prevalence of extended-spectrum -lactamase producing enterobacteriaceae among clinical isolates in Burkina Faso. BMC Infect Dis 2016;(16):326.
Jang J, Suh YS, Di DY, Unno T, Sadowsky MJ, Hur HG. Pathogenic Escherichia coli strains producing extended-spectrum β‐lactamases in the Yeongsan River Basin of South Korea. Environ Sci Technol 2012;47(2):1128-1136.
Colomer-Lluch M, Jofre J, Muniesa M. Quinolone resistance genes (qnrA and qnrS) in bacteriophage particles from wastewater samples and the effect of inducing agents on packaged antibiotic resistance genes. J Antimicrob Chemother 2014;69(5):1265-1274.
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