Molecular characterization of Staphylococcus spp. isolates associated with bovine mastitis in Tarimbaro, Michoacan, Mexico

Authors

  • Joel Edmundo López Meza
  • Jorge Ernesto Higuera Ramos
  • Alejandra Ochoa Zarzosa
  • Omar Chassin Noria
  • Juan José Valdez Alarcón
  • Alejandro Bravo Patiño
  • Víctor Manuel Baizabal Aguirre

Keywords:

Antibiotic resistance

Abstract

Mastitis is a disease of dairy cattle worldwide that causes decreased milk quality/yield, resulting in major economic losses. Mastitis is caused by a wide variety of organisms, including Staphylococcus aureus as a major pathogen. The purpose of this research was to characterize 20 Staphylococcus spp. isolates from the mammary gland of mastitic, lactating, backyard cattle, using both the Random Amplified Polymorphic DNA (RAPD) analysis, and sensitivity tests to 13 antibiotics and 1 detergent. Sensitivity tests to 13 antibiotics showed that all isolates were resistant to penicillin but sensitive to cephalothin, erythromycin, and trimethoprim. All isolates were susceptible to a <6 µg/ml cethyl-trimethyl ammonium bromide (CTAB) concentration. Molecular characterization aided to determine that isolates SA7 & SA8, SA30 & SA32, showed >90% similarity in the RAPD band pattern, while isolates SA35 & SA36 were identical to each other. Nevertheless, all other isolates showed different amplification profiles. Isolates SA7 & SA8, SA30 & SA32, SA35 & SA36 had similar antibiotic resistance/RAPD patterns, suggesting re-isolation. Staphylococcus isolates collected in Tarímbaro, Michoacán showed several different amplification patterns.

Downloads

Published

2012-01-01

How to Cite

López Meza, J. E., Higuera Ramos, J. E., Ochoa Zarzosa, A., Chassin Noria, O., Valdez Alarcón, J. J., Bravo Patiño, A., & Baizabal Aguirre, V. M. (2012). Molecular characterization of Staphylococcus spp. isolates associated with bovine mastitis in Tarimbaro, Michoacan, Mexico. Revista Mexicana De Ciencias Pecuarias, 44(1), 91 a 106. Retrieved from https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1757
Metrics
Views/Downloads
  • Abstract
    788
  • PDF (Español)
    575

Issue

Section

Articles

Metrics

Similar Articles

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)