Polymorphisms associated with the number of live-born piglets in sows infected with the PRRS virus in southern Sonora Mexico

Autores/as

  • Carlos Martín Aguilar-Trejo Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.
  • Guillermo Luna-Nevárez Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.
  • Javier Rolando Reyna-Granados Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.
  • Ricardo Zamorano-Algandar Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.
  • Javier Alonso Romo-Rubio Universidad Autónoma de Sinaloa, Facultad de Medicina Veterinaria y Zootecnia, Culiacán Sinaloa, México.
  • Miguel Ángel Sánchez-Castro Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.
  • R. Mark Enns Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.
  • Scott E. Speidel Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.
  • Milton G. Thomas Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.
  • Pablo Luna-Nevárez Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.

DOI:

https://doi.org/10.22319/rmcp.v11i3.5002

Palabras clave:

alleles, breeding sows, genotype, live-born, piglets, PRRS, SNP

Resumen

The porcine reproductive and respiratory syndrome (PRRS) is a viral disease that decreases the reproductive performance in breeding sows and leads to economic losses to the swine industry. The objective of the present study was to identify single nucleotide polymorphisms (SNP) associated to the number of live-born piglets in the first (LBP1) and second birth (LBP2) in breeding sows exposed to PRRS virus. The study included 100 pregnant females of the Landrace(¾)/ Yorkshire(¼) line, 75 of which were infected with the PRRS virus and 25 were free of PRRS. Individual blood samples (6-8 drops) were obtained and spotted onto FTA cards and subsequently processed for DNA extraction, which was genotyped using a 10,000 SNP chip for genomic profile. Resulting genotypes were analyzed using a multi-locus mixed model that detected three SNP associated to LBP1 and five SNP associated to LBP2 (P<0.001). These eight SNP were validated using an associative mixed effects model which included the terms genotype and age of dam as fixed effects, and sire as random effect. Allele substitution effects were estimated using the same model including the term genotype as covariate. The SNP rs81276080, rs81334603 and rs80947173 were associated to LBP1 (P<0.001), whereas the SNP rs81364943, rs80859829, rs80895640, rs80893794 and rs81245908 were associated to LBP2 (P<0.001). Only two SNP were in functional chromosomal regions and the remainder SNP were within an intergenic position. In conclusion, these results suggest the existence of gene variants associated with the reproductive performance of sows infected with the PRRS virus.

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Biografía del autor/a

Carlos Martín Aguilar-Trejo, Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.

Departamento de Ciencias Agronómicas y Veterinarias

Profesor-Investigador

Guillermo Luna-Nevárez, Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.

Departamento de Ciencias Agronómicas y Veterinarias

Investigador Colaborador

Javier Rolando Reyna-Granados, Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.

Vice-rectoría Administrativa

Vice-rector

Javier Alonso Romo-Rubio, Universidad Autónoma de Sinaloa, Facultad de Medicina Veterinaria y Zootecnia, Culiacán Sinaloa, México.

Colegio en Ciencias Agropecuarias

Coordinador de Posgrado

Miguel Ángel Sánchez-Castro, Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.

Department of Animal Sciences

PhD. Student

R. Mark Enns, Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.

Department of Animal Sciences 

Professor

Scott E. Speidel, Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.

Department of Animal Sciences 

Associate Professor

Milton G. Thomas, Colorado State University, Department of Animal Sciences, Fort Collins Colorado, USA.

Department of Animal Sciences

Professor

Pablo Luna-Nevárez, Instituto Tecnológico de Sonora, Departamento de Ciencias Agronómicas y Veterinarias, Calle 5 de Febrero # 818 Sur, Colonia Centro, Ciudad Obregón Sonora, México.

Departamento de Ciencias Agronómicas y Veterinarias

Profesor-Investigador

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Publicado

21.09.2020

Cómo citar

Aguilar-Trejo, C. M., Luna-Nevárez, G., Reyna-Granados, J. R., Zamorano-Algandar, R., Romo-Rubio, J. A., Sánchez-Castro, M. Ángel, … Luna-Nevárez, P. (2020). Polymorphisms associated with the number of live-born piglets in sows infected with the PRRS virus in southern Sonora Mexico. Revista Mexicana De Ciencias Pecuarias, 11(3), 828–847. https://doi.org/10.22319/rmcp.v11i3.5002
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