Detection of rabies virus in organs unrelated to the central nervous system of experimentally-inoculated vampire bats

Autores/as

  • María Luisa Méndez-Ojeda Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ciudad de México. México. Instituto de Neuroetología - Universidad Veracruzana, Xalapa, Veracruz. México.
  • Edith Rojas-Anaya Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ciudad de México. México.
  • Jose Francisco Morales-Álvarez Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ciudad de México. México.
  • Graciela Tapia-Pérez Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México. México.
  • Gerardo Suzán Departamento de Etología, Fauna Silvestre y Animales de Laboratorio, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México. Ciudad de México. México.
  • Osiris Gaona Pineda Departamento de Ecología y Conservación de Vertebrados, Instituto de Ecología, Universidad Nacional Autónoma de México. Ciudad de México. México.
  • Rodrigo A. Medellín-Legorreta Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP
  • Charles E Rupprecht Lyssa LLC. Lawrenceville, Atlanta, Georgia. USA.
  • Elizabeth Loza-Rubio Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Ciudad de México. México.

DOI:

https://doi.org/10.22319/rmcp.v9i3.4247

Palabras clave:

Desmodus rotundus, Pathogenesis, Rabies, Vampire bat, Zoonosis.

Resumen

The aim of this research was to detect rabies virus in peripheral tissues in captive vampires. Vampire bats were inoculated with 106 MICLD50 of homologous rabies virus. Bats displayed clinical signs of rabies beginning on d 8 until the 19th d post-inoculation (pi). Rabies virus antigens were found in the brain of all rabid bats. Viral RNA was detected in brain, salivary gland and tongue tissue by RT-PCR and nested PCR (nPCR). Viral genome was also detected in organs unrelated to the central nervous system. Rabies virus was not detected in saliva nor documented from any tissues without occurrence of viral antigens in the brain. Host humoral response was most pronounced via the induction of viral neutralizing antibodies (VNA) from d 8 to 20 pi, having a peak at d 14 with 0.9 IU. Antibody levels were variable, but tended to remain high after inoculation, showing significant differences to the negative control group (P=0.001). This research is one of the few recent studies focused upon Desmodus rotundus and contributes to the basic knowledge of rabies virus pathogenesis, which is required for an understanding of perpetuation in a major viral reservoir in Latin America.

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Biografía del autor/a

Rodrigo A. Medellín-Legorreta, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, INIFAP

INVESTIGADOR TITULAR C, RESPONSABLE DEL DEPARTAMENTO DE BIOTECNOLOGÍA EN SALUD ANIMAL DEL CENID-MICROBIOLOGÍA ANIMAL, INIFAP

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Publicado

29.06.2018

Cómo citar

Méndez-Ojeda, M. L., Rojas-Anaya, E., Morales-Álvarez, J. F., Tapia-Pérez, G., Suzán, G., Gaona Pineda, O., … Loza-Rubio, E. (2018). Detection of rabies virus in organs unrelated to the central nervous system of experimentally-inoculated vampire bats. Revista Mexicana De Ciencias Pecuarias, 9(3), 435–450. https://doi.org/10.22319/rmcp.v9i3.4247
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