Intracellular survival of Mycobacterium bovis strains with high and low frequency in cattle populations in a bovine macrophage model

Autores/as

  • Alejandro Nava-Vargas Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales, Doctorado en Ciencias Biológicas, Querétaro, México.
  • Feliciano Milián-Suazo Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales, Querétaro, México.
  • Germinal Jorge Cantó-Alarcón Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales, Querétaro, México.
  • José A. Gutiérrez-Pabello Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Av. Universidad #3000, Colonia, C.U., Coyoacán, 04510, Ciudad de México, México.

DOI:

https://doi.org/10.22319/rmcp.v12i2.5542

Palabras clave:

Mycobacterium bovis, Bovine tuberculosis, Macrophages

Resumen

Bovine tuberculosis is a disease caused by Mycobacterium bovis that affects cattle and other species, including humans. Mycobacterium bovis resides mainly in macrophages, so bacilli survival within macrophages is related to virulence. Isolation and strain identification are important for disease control. However, little is known about virulence of the circulating strains in cattle populations. Therefore, the aim of this study was to compare the intracellular survival of Mycobacterium bovis strains with high and low frequency genotypes in cattle in Mexico. Four high frequency genotypes and four low frequency genotypes were identified and subjected to intracellular survival assays in bovine macrophages. Results showed that the phagocytosis proportion was approximately 63 % for all strains. There were no significant differences in the average Colony Forming Units (CFUs) in phagocytosis and survival between the high and low frequency groups; however, when the CFU average of phagocytosis was compared with the survival, significant differences were found in both groups. In intracellular growth, a significant difference was observed between low and high frequency strains, and between low frequency strains. Finally, the intracellular growth average of the groups was analyzed showing no significant difference. These results suggest that the frequency of the genotype in cattle population is not related to the intracellular survival and the virulence of the M. bovis strains.

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Biografía del autor/a

Germinal Jorge Cantó-Alarcón, Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales, Querétaro, México.

Secretario Gneral

Facultad de Medicina Veterinaria y Zootecnia

Citas

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Publicado

15.09.2021

Cómo citar

Nava-Vargas, A., Milián-Suazo, F., Cantó-Alarcón, G. J., & Gutiérrez-Pabello, J. A. (2021). Intracellular survival of Mycobacterium bovis strains with high and low frequency in cattle populations in a bovine macrophage model. Revista Mexicana De Ciencias Pecuarias, 12(2), 487–502. https://doi.org/10.22319/rmcp.v12i2.5542
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