Aislamiento e identificación de bacterias ácido lácticas con potencial probiótico para becerros del altiplano mexicano
DOI:
https://doi.org/10.22319/rmcp.v10i1.4512Palabras clave:
Becerras, Probióticos, Aislamiento, Resistencia.Resumen
Los becerros neonatos son expuestos continuamente a un amplio rango de microorganismos habitantes del ambiente, y a patógenos causantes de diarrea. El objetivo de este estudio fue aislar e identificar bacterias acido lácticas (BAL) de la mucosa oral de becerros, calostro y leche de vacas Holstein. El aislamiento de BAL se realizó en caldo y placas con medio ManRogosa Sharp (MRS). Una vez purificadas las colonias bacterianas, se realizaron pruebas morfológicas y bioquímicas para la caracterización de BAL. Se aislaron 16 colonias bacterianas, de las cuales se clasificaron en 12 de la mucosa oral, 2 en leche y 2 en calostro. Estas colonias se evaluaron a pH ácido (4.0 y 4.5) y sales biliares (SB: 0.3 y 1.5 g). Posteriormente se identificaron con el Sistema API50CHL. Las especies aisladas con resistencia a pH de 4 y 1.5 de SB fueron: Leuconostoc mesenteroides, Pediococcus pentosaceus, Lactobacillus plantarum. Lactobacillus crispatus y Lactococcus lactis. Estas colonias cuentan con un potencial probiótico para ser usadas en becerros.
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