Effect of viscosity on the medium for rooster (Gallus gallus) sperm cryopreservation
DOI:
https://doi.org/10.22319/rmcp.v13i1.5917Keywords:
Acrosome, Sperm capacitation, Freezing, Gallus gallus, SemenAbstract
In mammalian semen, viscosity has been shown to have a negative influence on its conservation. In bird semen, studies on the physical characteristics of ejaculates are limited, particularly viscosity has not been studied. The media for cryopreservation do not consider viscosity to maintain sperm viability. Therefore, the objective of this study was to evaluate the effect of viscosity on the medium to maintain its viability after thawing. The parameters of basic evaluation, maturation and acrosome reaction were determined, evaluating the presence and distribution of Ca2+ through co-incubation with chlortetracycline. Twenty-five (25) evaluations of seminal pool were performed, cryopreserved in Lake medium supplemented with 6 % dimethylacetamide and with 0 % (Control), 10 %, 30 % and 45 % ficoll, to adjust the viscosity of the medium to conditions similar to those of semen and oviductal fluid and to a higher degree of viscosity. Sperm motility was lower (P£0.05) in aliquots with a higher percentage of ficoll. The percentage of live spermatozoa was similar (P>0.05) in the control and all aliquots with different percentage of ficoll. Sperm maturation presented a higher (P£0.05) percentage of non-capacitated spermatozoa when 10 % ficoll was used. Conversely the percentage of spermatozoa with acrosome reaction was also lower (P£0.05) when 10 % ficoll was used. The results of this study show that variations in the degree of viscosity of the medium can maintain or increase sperm viability after thawing.
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Herrera JA, Quintana JA, López M, Betancourt M, Fierro R. Individual cryopreservation with dimethyl sulfoxide and polyvinylpyrrolidone of ejaculates and pooled semen of three avian species. Arch Androl 2005;51(5):353-360. doi: 10.1080/014850190944401.
Bootwalla S. Froman D. Effect of extender viscosity on the insemination dose for chickens. Poult Sci 1988;67(8):1218-1221. doi: 10.3382/ps.0671218.
Adams G, Ratto M, Collins C, Bergfelt D. Artificial insemination in South American camelids and wild equids. Theriogenology 2009;71(1):166-175. doi: 10.1016/j.theriogenology.2008.09.005.
Bravo P, Alarcon V, Baca L, Cuba Y, Ordonez C, Salinas J, Tito, F. Semen preservation and artificial insemination in domesticated South American camelids. Anim Rep Sci 2013;136(3):157-163. doi: 10.1016/j.anireprosci.2012.10.005.
El-Bahrawy K, Rateb S, Khalifa M, Monaco D, Lacalandra G. Physical and kinematic properties of cryopreserved camel sperm after elimination of semen viscosity by different techniques. Anim Rep Sci 2017;187(sn):100-108. doi: 10.1016/j.anireprosci.2017.10.011.
Holt W. Germoplasm cryopreservation in elephants and wildt ungulates. In: Cryobanking the genetic resource. Wildlife conservation for the future? Watson PF, Holt W, editors. CRC Press. 2001:319-348.
Baskt M, Wishart G, Brillard J. Oviductal sperm selection, transport, and storage in poultry. Poult Sci Rev 1994;5(3):117-143.
Blesbois E, Brillard J. Specific features of in vivo and in vitro sperm storage in birds. Animal 2007;1(10):1472-1481. doi: 10.1017/S175173110700081X.
Baskt M, Bauchan G. Lectin staining of the uterovaginal junction and sperm-storage tubule epithelia in broiler hens. Poult Sci 2016;95(4):948-955. doi.org/10.3382/ps/pev440.
Santiago-Moreno J, Esteso M, Villaverde-Morcillo S, Toledano-Diaz A, Castano C, Velázquez R, et al. Recent advances in bird sperm morphometric analysis and its role in male gamete characterization and reproduction technologies. Asian J Androl 2016;18(6):882-888. doi: 10.4103/1008-682X.188660.
Lemoine M, Grasseau I, Brillard J, Blesbois E. A reappraisal of the factors involved in vitro initiation of the acrosome reaction in chicken spermatozoa. Reproduction 2008;136(4):391. doi:10.1530/REP-08-0094.
Longobardi V, Albero G, De Canditiis D, Salzano A, Natale A, Balestrieri A, Neglia G, Campanile G, Gasparrini B. Cholesterol-loaded cyclodextrins prevent cryocapacitation damages in buffalo (Bubalus bubalis) cryopreserved sperm. Theriogenology 2017;89(sn):359–364. doi: 10.1016/j.theriogenology.2016.09.048
Laffaldano N, Di Iorio M, Cerolini S, Manchisi A. Overview of turkey semen storage: focus on cryopreservation. Annals Anim Sci 2016;16(4):961-974. https://doi.org/10.1515/aoas-2016-0026.
Norma Oficial Mexicana NOM-062-ZOO-1999. Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio.
Burrows W, Quinn J. The collection of spermatozoa from the domestic fowl and turkey. J Poult Sci 1937;16(1):19-24. https://doi.org/10.3382/ps.0160019.
Lake P. The principles and practice of semen collection and preservation in birds. In: Symposia of the Zoological Society of London. 1978;43:31–49.
Camarillo R, Jiménez I, Guzmán A, Rosales A, Rodríguez F, Pérez-Rivero J, Herrera J. Oviductal proteins effect in rooster spermatic cryopreservation. CryoLetters 2019; 40(6): 352-356. http://www.cryoletters.org/Abstracts/vol_40_6_2019.htm.
Fischer DH, Failing SK, Meinecke‐Tillmann S, Wehrend A, Lierz M. Viability assessment of spermatozoa in large falcons (Falco spp.) using various staining protocols. Reprod Dom Anim 2020;55(10):1383-1392. https://doi.org/10.1111/rda.13785.
Lemoine M, Mignon S, Grasseau I, Magistrini M, Bleisbois E. Ability of chicken spermatozoa to undergo acrosome reaction after liquid storage or cryopreservation. Theriogenology 2011;75(1):122-130. doi: 10.1016/j.theriogenology.2010.07.017.
Herrera JA, Calderón G, Guzmán A, Vargas AK, Ávalos A, Rosales A. Evaluation of two diluents for the storage of fresh and cryopreserved eyaculado of Harris hawk (Parabuteo unicinctus). Autrals J Vet Sci 2017;49(1):39-43. doi.org/10.4067/s0719-81322017000100039.
Hammer Ø, Harper D, Ryan P. PAST: Paleontological statistics software package for education and data analysis. Paleontología Electrónica 2001;4(1):1-9.
Donoghue A, Wishart G. Storage of poultry semen. Anim Rep Sci 2000;62(1-3):213- 232. doi: 10.1016/s0378-4320(00)00160-3.
Skidmore J. The use of some assisted reproductive technologies in old world camelids. Anim Rep Sci 2019; 207(sn):138-145. doi: 10.1016/j.anireprosci.2019.06.001.
Al-Bulushi S, Manjunatha B, Bathgate R, Rickard J, De Graaf S. Liquid storage of dromedary camel semen in different extenders. Anim Rep Sci 2019; 207(sn):95-106. https://doi.org/10.1016/j.anireprosci.2019.06.008.
Lemoine, M. Potential involvement of several signaling pathways in initiation of the chicken acrosome reaction. Biol Rep 2009;81(4):657–665. doi: 10.1095/biolreprod.108.072660.
Ali M, Musa M, Alfadul S, Al-Sobayel K. Effect of gum arabic on stallion sperm survival during cold storage and post freezing. Mac Vet Rev 2018;41(1):21-31. https://doi.org/10.1515/macvetrev-2017-0026.
Lemoine M, Grasseau I, Brillard JP, Blesbois E. A reappraisal of the factors involved in in vitro initiation of the acrosome reaction in chicken spermatozoa. Reproduction 2008;136(4):391-399. doi: 10.1530/REP-08-0094.
Abouelezz F, Sayed M, Santiago-Moreno J. Fertility disturbances of dimethylacetamide and glycerol in rooster sperm diluents: Discrimination among effects produced pre and post freezing-thawing process. Anim Rep Sci 2017;184(sn):228-234. DOI: 10.1016/j.anireprosci.2017.07.021.
Mocé E, Grasseau I, Blesbois E. Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react. Anim Rep Sci 2010;122(3-4):359-366. doi: 10.1016/j.anireprosci.2010.10.010.
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