Resistencia antimicrobiana de Gallibacterium anatis aisladas de gallinas de postura comercial en Sonora, México
DOI:
https://doi.org/10.22319/rmcp.v8i3.4506Palabras clave:
Gallibacterium anatis, PCR, Resistencia, Susceptibilidad, Antimicrobianos.Resumen
Se consiguieron identificar 23 aislamientos de Gallibacterium anatis, a través de pruebas convencionales de cultivo y bioquímicas, confirmándose su identificación a través del método de PCR. Se les efectuó la prueba de difusión en placa para determinar la resistencia antimicrobiana a los antibióticos más utilizados en el área avícola. Los aislamientos fueron más susceptibles a ceftiofur (73 %) y florfenicol (68 %); todos los aislamientos mostraron resistencia marcada a penicilina, tilosina, lincomicina, ampicilina, enrofloxacina, oxitetraciclina, norfloxacina y cefalexina. La presencia de Gallibacterium anatis se asocia a problemas respiratorios y reproductivos en las poblaciones de gallinas de postura comercial. Los aislamientos mostraron una resistencia marcada a distintos antibióticos, probablemente por la medicación desmedida ante la presencia de este microorganismo.
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Mirle C, Schoengarth M, Meinhart H, Olm U. Studies into the incidence of Pasteurella haemolytica infections and their relevance to hens, with particular reference to diseases of the egg-laying apparatus. Monat Vet 1991;(45):545-549.
Bager RJ, Persson G, Nesta B, Soriani M, Serino L, Jeppsson M. Outer membrane vesicles reflect environmental cues in Gallibacterium anatis. Vet Microbiol 2013;167(3-4):565-572.
Bojesen AM, Nielsen SS, Bisgaard M. Prevalence and transmission of haemolytic Gallibacterium species in chicken production systems with different biosecurity levels. Avian Pathol 2003;32(5):503-510.
Mirle C, Schöngarth M, Meinhart H, Olm U. Studies into the incidence of Pasteurella haemolytica infections and their relevant to hens, with particular reference to diseases of the egg-laying apparatus. Monatshefte für Veterinärmedizin 1991;(45):545-549.
Mushin R, Weisman Y, Singer N. Pasteurella haemolytica found in respiratory tract of fowl. Avian Dis 1980;24(1):162-168.
Rzewuska M, Karpiñska E, Szeleszczuk P, Binek M. Isolation of Gallibacterium spp. from peacocks with respiratory tract infections. Med Weter 2007;63(11):1431-1433.
Kristensen BM, Frees D, Bojesen AM. GtxA from Gallibacterium anatis, a cytolytic RTX-toxin with a novel domain organization. Vet Res 2010;(41):25-29.
García-Gómez E, Vaca S, Pérez-Méndez A, Ibarra-Caballero J, Pérez Márquez V, Tenorio VR. Gallibacterium anatis secreted metalloproteases degrade chicken IgG. Avian Pathol 2005;(34):426-429.
Bager RJ1, Nesta B, Pors SE, Soriani M, Serino L, Boyce JD, Adler B, Bojesen AM. The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate. Infect Immun 2013; 81(6):1964-1973.
Bisgaard M. Incidence of Pasteurella haemolytica in the respiratory tract of apparently healthy chickens and chickens with infectious bronchitis. Characterization of 213 strains. Avian Pathol 2007;6(4):285-292.
Alispahic M, Christensen H, Hess C, Razzazi-Fazeli E, Bisgaard M, Hess M. MALDI-TOF mass spectrometry confirms clonal lineages of Gallibacterium anatis between chicken flocks. Vet Microbiol 2012;(160):269-273.
Paudel S, Alispahic M, Liebhart D, Hess M, Hess C. Assessing pathogenicity of Gallibacterium anatis in a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization. Avian Pathol 2013;(42):6-12.
Aarestrup FM, Seyfarth AM, Angen O. Antimicrobial susceptibility of Haemophilus parasuis and Histophilus somni from pigs and cattle in Denmark. Vet Microbiol 2004;101(2):143-146.
Aarestrup FM. Veterinary drug usage and antimicrobial resistance in bacteria of animal origin. Basic Clin Pharmacol Toxicol 2005;(96):279-285.
Bojesen AM, Vázquez ME, Bager RJ, Ifrah D, González C, Aarestrup FM. Antimicrobial susceptibility and tetracycline resistance determinant genotyping of Gallibacterium anatis. Vet Microbiol 2011;148(1):105-110.
Unión de Asociaciones Avícolas del Estado de Sonora (UAAES). 1999.
Lowenstine LJ. Necropsy procedures. In: Harrison GJ, Harrison LR editors. Clinical avian medicine and surgery. USA: WB Saunders Co; 1986;23:298-309.
Woodford MH, Keet DF, Bengis RG. Postmortem procedures for wildlife veterinarians and field biologists. Published jointly by the Office International des Epizooties, Care for the Wild and the Veterinary Specialist Group/Species Survival Commission of the World Conservation Union (IUCN) 2000;54-55.
Christensen H, Bisgaard M, Bojesen AM, Mutters R, Olsen JE. Genetic relationships among avian isolates classified as Pasteurella haemolytica, “Actinobacillus salpingitidis” or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov. Int J Syst Evol Microbiol 2003;(53):275-287.
Rimler RB, Sandhu TS, Glisson JR. Pasteurellosis, infectious serositis, and pseudotuberculosis. In: A laboratory manual for the Isolation and Identification of avian pathogens. Swayne DE editor. Philadelphia: American Association of Avian Pathogens; 1998.
DGRM-01. Guía rápida de operación con sistema LABPRO. DICIPA. 2006.
Bisgaard M. Isolation and characterization of some previously unreported taxa from poultry with phenotypical characters related to Actinobacillus and Pasteurella species. Acta Patholo Microbiol Immunol 1982;(90):59–67.
Bojesen AM, Vázquez ME, Robles F, González C, Soriano EV, Olsen JE, Christensen H. Specific identification of Gallibacterium by a PCR using primers targeting the 16S rRNA and 23S rRNA genes. Vet Microbiol 2007;123(1-3):262-268.
Lane DJ. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow MN editors. Nucleic acid techniques in bacterial systematics. Chichester United Kingdom: John Wiley and Sons; 1991.
National Committee for Clinical Laboratory Standards (NCCLS). Performance standards for antimicrobial susceptibility testing: 12th Inform Suppl. Wayne (PA): NCCLS, Document M100-S12; 2002;22(1):15-39.
Barry AL, Thornsberry C. Susceptibility test diffusion test procedures. In: Lennette E editor. Manual of clinical microbiology, 4th ed. Am Soc Microbiol 1985;978-987.
Quinn PJ, Markey BK, Manguirre D. Elementos de la microbiología veterinaria. México: Acribia; 2003.
Bojesen AM, Torpgahl M, Christensen H, Olsen JE, Bisgaard M. Genetic diversity of Gallibacterium anatis Insolates from different chicken flocks. J Clin Microbiol 2003;41(6):2737-2740.
Tegtmeier C, Angen O, Ahrens P. Comparison of bacterial cultivation, PCR, in situ hybridization and immunohistochemistry as tools for diagnosis of Haemophilus somnus pneumonia in cattle. Vet Microbiol 2000;76(4):385-394.
Bojesen AM, Vázquez ME, Bager RJ, Ifrah D, González C, Aarestrup FM. Antimicrobial susceptibility and tetracycline resistance determinant genotyping of Gallibacterium anatis. Vet Microbiol 2011;148:105-110.
Bojesen AM, Bager RJ, Ifrah D, Aarestrup FM. The rarely reported tet (31) tetracycline resistance determinant is common in Gallibacterium anatis. Vet Microbiol 2011;149:497-499.
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