Fatty acids and terpenes from the methanolic extract of Artemisia cina as possible compounds responsible for the ovicidal effect on Haemonchus contortus

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DOI:

https://doi.org/10.22319/rmcp.v15i3.6468

Keywords:

Artemisia cina, Haemonchus contortus, Egg hatching, Anthelmintic

Abstract

Haemonchus contortus is a hematophagous nematode with a high reproduction rate, considered to be the main problem in grazing small ruminants. Therefore, treatment alternatives based on the use of plant extracts are sought. This study aimed to evaluate the ovicidal activity of Artemisia cina against the parasite Haemonchus contortus and to chemically characterize the extract with the highest biological activity through gas chromatography coupled with mass spectrometry (GC-MS). The extracts to be evaluated were obtained through the maceration technique using methanol, ethyl acetate, and n-hexane. The extracts were taken to total dryness and challenged against H. contortus eggs using the egg-hatching inhibition technique described by the World Association for the Advancement of Veterinary Parasitology (WAAVP). The methanolic extract (ME) showed 100 % ovicidal activity at a concentration of 4.25 mg/ml, being the most active at a low concentration; therefore, it was characterized by GC-MS. ME mainly contains fatty acids and terpenes; among them are hexadecanoic acid and 2-[4-methyl-6-(2,6,6-trimethylcyclohex-1-enyl)hexa-1,3,5-trienyl][cyclohex]-1-en-carboxyaldehyde. The characterized compounds have shown previously reported anthelmintic activity so that ovicidal activity may be associated with them. In conclusion, the methanolic extract of A. cina had a higher ovicidal activity at low concentrations; this is probably due to the presence of fatty acids and terpenes.

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References

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Published

2024-08-12

How to Cite

Arango-De-la-Pava, L. D., De la Cruz-Cruz, H. A., Cuéllar-Ordaz, J. A., Zamilpa, A., González-Cortázar, M., López-Arellano, M. E., … López-Arellano, R. (2024). Fatty acids and terpenes from the methanolic extract of Artemisia cina as possible compounds responsible for the ovicidal effect on Haemonchus contortus. Revista Mexicana De Ciencias Pecuarias, 15(3), 734–748. https://doi.org/10.22319/rmcp.v15i3.6468
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Vol. 15 No. 3 (2024): July-September

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Research Notes

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