Evaluación antihelmíntica in vitro de curcumina contra huevos y larvas de aislados de Haemonchus contortus con distinta susceptibilidad a ivermectina
DOI:
https://doi.org/10.22319/rmcp.v15i4.6440Palabras clave:
polifenol, antihelmíntico, desenvaine larvario, estabilizadores poliméricosResumen
El creciente problema de resistencia a los antihelmínticos (AHs) convencionales en poblaciones de nematodos gastrointestinales (NGI), ha motivado la búsqueda de alternativas para su control en los rebaños de ovinos y caprinos, como es el caso de los metabolitos secundarios de plantas. En el presente trabajo se evaluó la actividad AH in vitro de curcumina (CUR) contra tres aislados del nematodo de rumiantes Haemonchus contortus con diferente susceptibilidad a los AHs convencionales. Se utilizaron las pruebas in vitro de inhibición de la eclosión de huevos (IEH), inhibición de la migración larvaria (IML), inhibición del desenvaine larvario (IDL) y mortalidad a las 72 h de L3 sin vaina. Las pruebas se realizaron usando un intervalo de concentraciones de 0 - 8.5 μg CUR/ml. Solamente la prueba de IDL fue realizada con un intervalo de concentración de 0 - 17.3 μg CUR/ml. Se obtuvieron las curvas de concentración-respuesta en una regresión log-logística y se realizó un diseño completamente al azar mediante un ANOVA. La CUR no presentó actividad AH en las pruebas de IEH, IML y mortalidad, pero sí demostró efecto AH significativo en la prueba de IDL. Este efecto fue más potente contra el aislado FMVZ-UADY (CE50= 1.9 μg/ml, IC95% 1.58-2.31), seguido por el aislado Paraíso (CE50= 3.2 μg/ml, IC95% 2.69-3.81) y CENID-SAI, INIFAP (CE50= 7.0 μg/ml; IC95% 6.58-7.43). Se concluye que la CUR tiene efecto AH contra el desenvaine de las L3 de H. contortus pero no afecta la eclosión de huevos, la migración de L3 o la mortalidad de L3 sin vaina a las dosis evaluadas.
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