Impact of an herbal additive on fermentation and rumen microbiota of lambs

Autores/as

  • Mario Iván Ávila-Estrada Universidad Autónoma Metropolitana-Xochimilco. División de Ciencias Biológicas y de la Salud. Doctorado en ciencias agropecuarias. Coyoacán, México City 04960, México. https://orcid.org/0009-0002-8252-1063
  • Germán David Mendoza-Martínez Universidad Autónoma Metropolitana-Xochimilco. Departamento de Producción Agrícola y Animal. México. https://orcid.org/0000-0002-8613-6464
  • Ismael Martínez-Cortés Universidad Autónoma Metropolitana-Xochimilco. Departamento de Producción Agrícola y Animal. México.
  • María Eugenia De la Torre-Hernández Universidad Autónoma Metropolitana-Xochimilco. Departamento de Producción Agrícola y Animal. México. | SECIHTI-Universidad Autónoma Metropolitana-Xochimilco. México. https://orcid.org/0000-0002-9982-2367
  • Hortencia Hernández-Ruíz Universidad Autónoma Metropolitana-Xochimilco. Departamento del Hombre y su Ambiente. México.
  • Hectro Aarón Lee-Rangel Universidad Autónoma de San Luis Potosí. Facultad de Agronomía y Veterinaria. México. https://orcid.org/0000-0001-6146-5790
  • Rogelio Flores-Ramírez Universidad Autónoma de San Luis Potosí. Facultad de Agronomía y Veterinaria. México. https://orcid.org/0000-0003-2263-6280
  • Augusto César Lizarazo-Chaparro Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia. México. https://orcid.org/0000-0001-9421-2087

DOI:

https://doi.org/10.22319/rmcp.v16i4.6840

Palabras clave:

Lambs, Bacteroidetes, Phytobiotic, Ocimum sanctum, Phyllanthus emblica

Resumen

The use of phytobiotics has shown beneficial changes in animal production and health due to the presence of secondary metabolites, some of which have vitamin C activity due to the antioxidant function, important in ruminants. This research evaluated the effects of a phytobiotic made with Phyllanthus emblica and Ocimum sanctum, which has shown vitamin C activity, on ruminal fermentation and microbiota. Ten (10) male Hampshire × Suffolk lambs were housed individually and randomly assigned to two groups: 0 or 15 g/kg of dry matter of the herbal additive for 60 d. Ruminal fluid samples were collected to determine volatile fatty acid concentrations by gas chromatography, and microbiota analysis was performed through DNA extraction and 16S rRNA gene V3-V4 region sequencing. Alpha diversity was analyzed using Chao1, Shannon, and Simpson’s reciprocal indices, while beta diversity was evaluated by principal coordinate analysis. Data were analyzed using an independent samples t-test and Pearson correlation. Supplementation decreased propionate concentration by 19.38 % (P= 0.05), with a tendency to increase the acetate:propionate ratio (P= 0.06), resulting in a 15.18 % increase in estimated methane concentration (P= 0.05), which affected fermentation efficiency. Regarding the microbiota, a 14.35 % decrease in Firmicutes (P= 0.06) and a 27.2 % increase in Bacteroidetes (P= 0.07) were observed. These results suggest that the phytobiotic modulates ruminal microbiota and fermentation.

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Publicado

18.11.2025

Cómo citar

Ávila-Estrada, M. I., Mendoza-Martínez, G. D., Martínez-Cortés, I., De la Torre-Hernández, M. E., Hernández-Ruíz, H., Lee-Rangel, H. A., … Lizarazo-Chaparro, A. C. (2025). Impact of an herbal additive on fermentation and rumen microbiota of lambs. Revista Mexicana De Ciencias Pecuarias, 16(4), 836–851. https://doi.org/10.22319/rmcp.v16i4.6840
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Vol. 16 Núm. 4 (2025): Octubre-Diciembre

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