Resveratrol-cyclodextrin complex during in vitro maturation improves bovine oocyte quality and enhances early embryonic development

Autores/as

  • Viviana Torres-Osorio Universidad CES. Facultad de Ciencias de la Salud. Grupo Ingennova. Medellín, Colombia.
  • Laura Muñoz-Sánchez Universidad CES. Facultad de Ciencias Agropecuarias y Naturales. Grupo INCA-CES. Medellín, Colombia.
  • Isabel C. Vélez Universidad CES. Facultad de Ciencias Agropecuarias y Naturales. Grupo INCA-CES. Medellín, Colombia.
  • Albeiro López-Herrera Universidad Nacional de Colombia. Facultad de Ciencias Agrarias. Grupo BIOGEM. Medellín, Colombia.
  • Rodrigo Urrego Universidad CES. Facultad de Ciencias Agropecuarias y Naturales. Grupo INCA-CES. Medellín, Colombia.

DOI:

https://doi.org/10.22319/rmcp.v17i1.6793

Palabras clave:

Antioxidant, Cyclodextrin, Embryonic development, In vitro maturation, Resveratrol

Resumen

Reactive oxygen species (ROS) may accumulate within oocytes during in vitro maturation (IVM) which is related to oocyte poor quality and decreased embryo development. Resveratrol (R) has been shown to protect against oxidative damage but has limited water solubility, so its inclusions with methyl-β-cyclodextrin (CD) improve its solubility. This study evaluated the effect of resveratrol-cyclodextrin (R-CD) complex during IVM on oxidative stress and their competence to become blastocyst after in vitro fertilization. In Experiment 1, cumulus oocyte complexes (COCs) from Bos indicus cattle were collected from a local slaughterhouse and matured in vitro either without (control) or with R-CD (1, 10, 20, 40 μM). The presence of ROS was assessed using 2′,7′-dichlorodihydrofluorescein diacetate staining, while glutathione (GSH) levels were determined using Cell Tracker Blue fluorescent staining. In Experiment 2, COCs were matured in vitro with R-CD, fertilized and cultured until d 7 to assess embryonic development. All the R-CD concentrations showed reduced intracellular ROS levels compared to control (P<0.01). Intracellular levels of GSH were significantly higher with R1-CD and R10-CD (P<0.01) compared to the other treatments. On the other hand, oocytes matured with R10-CD had a significantly higher blastocysts rate than other treatments. In conclusion, 10 μM R-CD complex during IVM improves bovine oocyte quality from Bos indicus by decreasing ROS levels and increasing intracellular GSH concentrations, resulting in greater blastocyst development.

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Citas

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Publicado

06.03.2026

Cómo citar

Torres-Osorio, V., Muñoz-Sánchez , L., Vélez, I. C., López-Herrera , A., & Urrego , R. (2026). Resveratrol-cyclodextrin complex during in vitro maturation improves bovine oocyte quality and enhances early embryonic development. Revista Mexicana De Ciencias Pecuarias, 17(1), e1716793. https://doi.org/10.22319/rmcp.v17i1.6793
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Vol. 17 Núm. 1 (2026): Enero-Marzo

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