Molecular follow-up study on the presence of bovine respiratory coronavirus in feedlot cattle in Northwest Mexico

Autores/as

  • Francisco Javier Monge-Navarro Universidad Autónoma de Baja California https://orcid.org/0000-0002-1857-0912
  • Soila Maribel Gaxiola Camacho Universidad Autónoma de Sinaloa. Facultad de Medicina Veterinaria y Zootecnia. Blvd. San Ángel 3886, Mercado de Abastos, San Benito. 80260 Culiacán Rosales, Sinaloa, México
  • Gilberto López Valencia Universidad Autónoma de Baja California. Instituto de Investigaciones en Ciencias Veterinarias. Mexicali, Baja California, México, México
  • Enrique Trasviña Muñoz Universidad Autónoma de Baja California. Instituto de Investigaciones en Ciencias Veterinarias. Mexicali, Baja California, México, México
  • Nohemí Castro Del Campo Universidad Autónoma de Sinaloa. Facultad de Medicina Veterinaria y Zootecnia. Blvd. San Ángel 3886, Mercado de Abastos, San Benito. 80260 Culiacán Rosales, Sinaloa, México
  • José Carloman Herrera Ramírez Universidad Autónoma de Baja California. Instituto de Investigaciones en Ciencias Veterinarias. Mexicali, Baja California, México, México

DOI:

https://doi.org/10.22319/rmcp.v16i3.6681

Palabras clave:

Coronavirus respiratorio bovino, ganado de engorda, qRT-PCR, prevalencia, carga viral

Resumen

In Mexico, studies on bovine respiratory coronavirus (BCoV) in feedlot cattle are scarce, and its presence has only been reported in feedlot cattle operations in the northwest of the country. The objective of this work was to conduct an epidemiological-molecular study in stabled feedlot cattle to establish the prevalence of BCoV and update the available information on its presence in the Northwest region of Mexico. Using quantitative real-time polymerase chain reaction (qRT-PCT) targeting a fragment of the gene that encodes for the spike protein of BCoV, 154 RNA samples from nasal exudate collected from asymptomatic animals (n=100) and sick (n= 54) were tested, from which 95 resulted positive for a general prevalence of 61.7 % and an average of 1 x 105 viral particles/ml of nasal exudate. Of them, 33 (21.4 %) belonged to the group of sick animals and 62 (40.3 %) to asymptomatic animals. The prevalence of BCoV established in this work demonstrates an increase of 23.7 percentage points above the prevalence of 38.0 % obtained in a previous study, making evident the necessity to introduce vaccination programs that confer protection against BCoV and help to control its expansion on the feedlot cattle of the region. It is also necessary to carry out ecological-epidemiological studies to establish the possible causes that favor the rapid spread of this virus among the feedlot cattle of Northwestern Mexico.

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Biografía del autor/a

Francisco Javier Monge-Navarro, Universidad Autónoma de Baja California

Investigador Titular C de Tiempo Completo, Definitivo.

Docotrado en Patología Comparada, Maestría en Salud Pública, Médico Veterinario Zootecnista.

Diagnóstico de Enfermedades, Virología, Microbiología, Biología Molecular, Proteómica, Zoonosis, Salud Pública, Una Salud, Bioseguridad, Bioterrorismo

Citas

Zhu Q, Su M, Li Z, Wang X, Qi S, Zhao F, et al. Epidemiological survey and genetic diversity of bovine coronavirus in Northeast China. Virus Res 2022;308:198632. https://doi.org/10.1016/j.virusres.2021.198632.

Mekata H, Hamabe S, Sudaryatma PE, Kobayashi I, Kanno T, Okabayashi T. Molecular epidemiological survey and phylogenetic analysis of bovine respiratory coronavirus in Japan from 2016 to 2018. J Med Vet Sci 2020;82(6):726–730. https://doi.org/10.1292/jvms.19-0587.

Oma VS, Klem T, Tråvén M, Alenius S, Gjerset B, Myrmel M, et al. Temporary carriage of bovine coronavirus and bovine respiratory syncytial virus by fomites and human nasal mucosa after exposure to infected calves. BMC Vet Res 2018;14(1):1-8. https://doi.org/10.1186/s12917-018-1335-1.

Hodnik JJ, Jezek JJ, Staric J. Coronaviruses in cattle. Trop Anim Health Prod 2020;52(6):2809-2016. doi:10.1007/s11250-020-02354-y.

Rodríguez CJL. Lopez VG, Monge NFJ, Medina BGE, Hori-Oshima S, Cueto GSA, et al. Detection and economic impact related to bovine respiratory disease, shrink, and traveling distance in feedlot cattle in Northwest Mexico. Turk J Vet Anim Sci 2017; 41(2):297-301. https://doi.org/10.3906/vet-1603-9.

Orozco CC, López VG, Muñoz-Del Real LM, Gaxiola CSM, Castro-del Campo N, Cueto GSA, et al. Molecular detection of bovine coronavirus associated with the bovine respiratory complex in beef cattle in the Mexicali Valley, Baja California, Mexico. Rev Mex Cienc Pecu 2020;11(4):933-945. https://doi.org/10.22319/rmcp.v11i4.5137.

Stokstad M, Klem TB, Myrmel M, Oma VS, Toftaker I, Østerås O, et al. Using biosecurity measures to combat respiratory disease in cattle: the Norwegian control program for bovine respiratory syncytial virus and bovine coronavirus. Front Vet Sci 2020;7:167. https://doi.org/10.3389/fvets.2020.00167.

Ellis J. What is the evidence that bovine coronavirus is a biologically significant respiratory pathogen in cattle? Can Vet J 2019;60(2):147-152. PMID: 30705449.

Smith G, Smith I, Harrower B, Warrilow D, Bletchly C. A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents. J Virol Methods 2006;135(2):229–234. https://doi.org/10.1016/j.jviromet.2006.03.007.

Cappellano G, Abreu H, Casale C, Dianzani U, Chiocchetti A. Nano-Microparticle platforms in developing next-generation vaccines. Vaccines 2021;9(6):606. https://doi.org/10.3390/vaccines9060606.

Parkhe P, Verma S. Evolution, interspecies transmission, and zoonotic significance of animal coronaviruses. Front Vet Sci 2021;8:719834. https://doi.org/10.3389/fvets.2021.719834.

Vlasova AN, Saif LJ. Bovine coronavirus and the associated diseases. Front Vet Sci 2021;8:643220. https://doi.org/10.3389/fvets.2021.643220.

Oma VS, Tråvén M, Alenius S, Myrmel M, Stokstad M. Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmission. Virol J 2016;13:1-11. https://doi.org/10.1186/s12985-016-0555-x.

Kanno T, Ishihara R, Hatama S, Uchida I. A long-term animal experiment indicating persistent infection of bovine coronavirus in cattle. J Vet Med Sci 2018;80(7):1134–1137. https://doi.org/10.1292/jvms.18-0050.

Zhang M, Hill JE, Alexander TW, Huang Y. The nasal viromes of cattle on arrival at western Canadian feedlots and their relationship to development of bovine respiratory disease. Transbound Emerg Dis 2021;68:2209–2218. https://doi.org/10.1111/tbed.13873.

Fulton RW, d’Offay JM, Landis C, Miles DG, Smith RA, Saliki JT, et al. Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease. Vaccine 2016;34(30):3478-3492. https://doi.org/10.1016/j.vaccine.2016.04.020.

McDaneld TG, Workman AM, Chitko-McKown CG, Kuehn LA, Dickey A, Bennett GL. Detection of Mycoplasma bovirhinis and bovine coronavirus in an outbreak of bovine respiratory disease in nursing beef calves. Front Microbiomes 2022;1:1051241. https://doi.org/10.3389/frmbi.2022.1051241.

Frucchi APS, Dall AAM, Bronkhorst DE, Beuttemmuller EA, Alfieri AA, Alfieri AF. Bovine coronavirus co-infection and molecular characterization in dairy calves with or without clinical respiratory disease. Front Vet Sci 2022;25;9:895492. doi: 10.3389/fvets.2022.895492.

O'Neill R, Mooney J, Connaghan E, Furphy C, Graham DA. Patterns of detection of respiratory viruses in nasal swabs from calves in Ireland: a retrospective study. Vet Rec 2014;175:351-351. https://doi.org/10.1136/vr.102574.

Studer E, Lutz S, Mireille M, Dimitri S, Ronald D, Melle H, et al. Prevalence of BRD-related viral pathogens in the upper respiratory tract of Swiss veal calves. Animals 2021;11(7):1940. https://doi.org/10.3390/ani11071940.

Paller T, Hostnik P, Pogačnik M, Toplak I. The prevalence of ten pathogens detected by a real-time PCR method in nasal swab samples collected from live cattle with respiratory disease. Slov Vet Zb 2017;54(3):101-107. UDC 636.2.09:616.2-002:616.214-076:577.21.

Pratelli A, Cirone F, Capozza P, Trotta A, Corrente M, Balestrieri A, et al. Bovine respiratory disease in beef calves supported long transport stress: An epidemiological study and strategies for control and prevention. Res Vet Sci 2021;135:450-455. https://doi.org/10.1016/j.rvsc.2020.11.002.

Brito BP, Frost MJ, Anantanawat K, Jaya F, Batterham T, Djordjevic SP, et al. Expanding the range of the respiratory infectome in Australian feedlot cattle with and without respiratory disease using metatranscriptomics. Microbiome 2023;11(1):158. https://doi.org/10.1186/s40168-023-01591-1.

Moore SJ, O’Dea MA, Perkins N, O’Hara AJ. Estimation of nasal shedding and seroprevalence of organisms known to be associated with bovine respiratory disease in Australian live export cattle. J Vet Diagn Invest 2015;27(1):6-17. doi:10.1177/1040638714559741.

Vlasova AN, Saif LJ. Bovine coronavirus and the associated diseases. Frontiers Vet Sci 2021;8,643220. https://doi.org/10.3389/fvets.2021.643220.

Bell RL, Turkington HL, Cosby SL. The bacterial and viral agents of BRDC: immune evasion and vaccine developments. Vaccines 2021;9,337. https://doi.org/10.3390/vaccines9040337.

Hasoksuz M, Hoet AE, Loerch SC, Wittum TE, Nielsen PR, Saif LJ. Detection of respiratory and enteric shedding of bovine coronaviruses in cattle in an Ohio feedlot. J Vet Diag Invest 2002;14:30813. doi:10.1177/104063870201400406.

Decaro N, Campolo M, Desario C, Cirone F, D’abramo M, et al. Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy. J Vet Diag Invest 2008;20:28–32. doi:10.1177/104063870802000105.

Ambrose RK, Blakebrough-Hall, C Gravel JL, Gonzalez LA, Mahony TJ. Characterisation of the upper respiratory tract virome of feedlot cattle and its association with bovine respiratory disease. Viruses 2023;15:455. https://doi.org/10.3390/v15020455.

Brito BP, Frost MJ, Anantanawat K, Jaya F, Batterham T, et al. Expanding the range of the respiratory infectome in Australian feedlot cattle with and without respiratory disease using metatranscriptomics. Microbiome 2023;11(1)58;2-17. https://doi.org/10.1186/s40168-023-01591-1.

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Publicado

28.08.2025

Cómo citar

Monge-Navarro, F. J., Gaxiola Camacho, S. M., López Valencia, G., Trasviña Muñoz, E., Castro Del Campo, N., & Herrera Ramírez, J. C. (2025). Molecular follow-up study on the presence of bovine respiratory coronavirus in feedlot cattle in Northwest Mexico. Revista Mexicana De Ciencias Pecuarias, 16(3), 661–676. https://doi.org/10.22319/rmcp.v16i3.6681
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Vol. 16 Núm. 3 (2025): Julio-Septiembre

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