Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig

Autores/as

  • Jorge Luis de la Rosa Arana Instituto de Diagnóstico y Referencia Epidemiológicos. Laboratorio de Inmunoparasitología. Ciudad de México. https://orcid.org/0000-0002-8460-5941
  • Jesús Benjamín Ponce Noguez Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.
  • Tzayhri Gallardo Velázquez Instituto Politécnico Nacional. Escuela Nacional de Ciencias Bilógicas, Departamento de Biofísica. Ciudad de México. https://orcid.org/0000-0002-9587-9091
  • Nydia Edith Reyes Rodríguez Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México. https://orcid.org/0000-0002-4307-8161
  • Andrea Paloma Zepeda Velázquez Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México. http://orcid.org/0000-0001-9289-9831
  • Ana Berenice López Lugo Universidad Nacional Autónoma de México. Facultad de Estudios Superiores Cuautitlán, laboratorio de Parasitología.
  • Alejandro Pablo Sánchez Paredes Universidad Nacional Autónoma de México. Facultad de Estudios Superiores Cuautitlán, laboratorio de Parasitología.
  • Pablo Martinez Labat Universidad Nacional Autónoma de México. Facultad de Estudios Superiores Cuautitlán, laboratorio de Parasitología.
  • Fabián Ricardo Gómez De Anda Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México. https://orcid.org/0000-0001-6314-099X

DOI:

https://doi.org/10.22319/rmcp.v12i4.5897

Palabras clave:

Trichinella, meat-inspection, sausage, diagnosis, infrared-spectroscopy

Resumen

The aim of this work was to determine the usefulness of Fourier transform infrared (FTIR) spectroscopy to detect muscle larvae of Trichinella spiralis (Owen, 1835) in ham and sausages made from the meat of an experimentally infected pig. It was searched for the muscle larvae (ML) by conventional methods (artificial digestion and trichinoscopy stained with Mayer´s hemalum stain) and by FTIR spectroscopy. In addition, the infective capacity of the larvae found in swine products was analyzed. The parasite load was 8.5 ± 3 mL/g in ham and 4.5 ± 1.4 mL/g in sausage (P<0.0001). The spectra of the pig products prepared with the meat of an uninfected pig were different in the range of 1,700 to 900 cm-1 with respect to the spectra of the products from infected pigs. In this region, glycogen is the most abundant chemical group (1,200 and 900 cm-1). The distance between classes between non-infested and infested products was 10.2 for ham and 5.52 for sausages (three is the minimum value to indicate class separation). The infective capacity of the larvae recovered from pig products decreased up to five times compared to that of the larvae obtained from experimentally infected mice. These results show that the FTIR spectroscopy is useful to determine the presence of T. spiralis larvae in the foods studied here. Further studies are needed to determine the influence of meat flavors on the detection of Trichinella by FTIR spectroscopy.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Ruetsch C, Delaunay P, Armengaud A, Peloux-Petiot F, Dupouy-Camet J, Vallée I, et al. Inadequate labeling of pork sausages prepared in Corsica causing a trichinellosis outbreak in France. Parasite 2016;23:27.

Ribicich M, Miguez M, Argibay T, Basso N, Franco A. Localization of Trichinella spiralis in muscles of commercial and parasitologic interest in pork. Parasite 2001;8(2 Suppl):S246-S248.

Pozio E, Pence DB, La Rosa G, Casulli A, Henke SE. Trichinella infection in wildlife of the southwestern United States. J Parasitol 2001;87(5):1208-1210.

Ortega-Pierres MG, Arriaga C, Yepez-Mulia L. Epidemiology of trichinellosis in Mexico, Central and South America. Vet Parasitol 2000;93(3-4):201-225.

Kapel CMO, Webster P, Gamble R. Muscle distribution of sylvatic and domestic Trichinella larvae in production animals and wildlife. Vet Parasitol 2005;132(1-2):101-105.

Noeckler K, Pozio E, van der Giessen J, Hill DE, Gamble HR. International Commission on Trichinellosis: Recommendations on post-harvest control of Trichinella in food animals. Food Waterborne Parasitol 2019;14:e00041.

Nöckler K, Pozio E, Voigt W, Heidrich J. Detection of Trichinella infection in food animals. Vet Parasitol 2000;93(3-4):335-350.

Konecsni K, Scheller C, Scandett B, Buholzer P, Gajadhar A. Evaluation of the PrioCHECK™ Trichinella AAD Kit for the digestion and recovery of larva in pork, horse meat and wild meat. Vet Parasitol 2017;243:267-271.

Gajadhar AA, Konecsni K, Scandrett B, Buholzer P. Validation of a new commercial serine protease artificial digestion assay for the detection of Trichinella larvae in pork. Food Waterborne Parasitol 2018;10:6-13.

Medina-Lerena MS, Ramirez-Álvarez A, Kühne M, Gómez-Priego A, de-la-Rosa JL. Influence of different processing procedures on the reproductive capacity of Trichinella spiralis in pork meat. Trop Anim Health Prod 2009;41(4):437-442.

Potter ME, Kruse MB, Matthews MA, Hill RO, Martin RJ. A sausage-associated outbreak of trichinosis in Illinois. Amer J Public Health 1976;66(12):1194-1196.

Hill DE, Luchanscky A, Porto-Fett A, Gamble R, Fournet VM, Hawkins-Cooper DS, et al. Curing conditions to inactivate Trichinella spiralis muscle larvae in ready-to-eat pork sausage. Food Waterborne Parasitol 2017;6:1-8.

Forbes LB, Measures L, Gajadhar A, Kapel C. Infectivity of Trichinella nativa in traditional northern (country) foods prepared with meat from experimentally infected seals. J Food Prot 2003;66(10):1857-1863.

Rohman A, Sismindari S, Erwanto Y, Che-Man YB. Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Sci 2011;88(1):91–95.

Sari TNI, Guntarti A. Wild boar fat analysis in beef sausage using Fourier Transform Infrared method (FTIR) combined with chemometric. Indonesian J Med Health 2018;9(1):16-23.

Gómez-de-Anda F, Gallardo-Velazquez T, Osorio-Revilla G, Dorantes-Alvarez L, Calderón-Domínguez G, Nogueda-Torres B, et al. Feasibility study for the detection of Trichinella spiralis in a murine model using mid-Fourier transform infrared spectroscopy (MID-FTIR) with attenuated total reflectance (ATR) and soft independent modelling of class analogies (SIMCA). Vet Parasitol 2012;190(3-4):496-503.

Gómez-de-Anda F, Dorantes-Álvarez L, Gallardo-Velazquez T, Osorio-Revilla G, Calderón-Domínguez G, Martínez-Labat P, et al. Determination of Trichinella spiralis in pig muscles using mid-Fourier transform infrared spectroscopy (MID-FTIR) with attenuated total reflectance (ATR) and soft independent modeling of class analogy (SIMCA). Meat Sci 2012;91(3):240-246.

Martínez-Fernández AR, Arribas B, Bolás F. Freezing resistance of spanish Trichinella isolates (T. spiralis and T. britovi). In: Gamble R, et al editors. Proc Ninth Int Conf Trichinellosis. 1rst ed. Ciudad de México, México: CINVESTAV; 1997:99-105.

Norma Oficial Mexicana NOM-062-ZOO-1999, Especificaciones técnicas para la Producción, Cuidado y Uso de los Animales de Laboratorio.

Norma Oficial Mexicana NOM-194-SSA1-2004, Productos y servicios. Especificaciones sanitarias en los establecimientos dedicados al sacrificio y faenado de animales para abasto, almacenamiento, transporte y expendio. Especificaciones sanitarias de productos.

Norma Oficial Mexicana NOM-213-SSA1-2002, Productos y servicios. Productos cárnicos procesados. Especificaciones sanitarias. Métodos de prueba.

Cooper DW. The preparation of serial sections of platyhelminth parasites, with details of the materials and facilities required. Syst Parasitol 1988;12:211-229.

de-la-Rosa JL, Álvarez N., Gómez-Priego A. Study of the reproductive capacity of Trichinella spiralis recovered from experimentally infected mice under-dosed with albendazole or mebendazole. Trop Biomed 2007;24(2):93-97.

Meza-Márquez OG, Gallardo-Velázquez T, Dorantes-Álvarez L, Osorio-Revilla G, de-la-Rosa-Arana JL. FT-MIR and Raman spectroscopy coupled to multivariate analysis for the detection of clenbuterol in murine model. Analyst 2011;136:3355-3365.

Lewis PD, Lewis KE, Ghosal R, Bayliss S, Lloyd AJ, Wills J, et al. Evaluation of FTIR spectroscopy as a diagnostic tool for lung cancer using sputum. BMC Cancer 2010;10:640.

Rohman A, Sismindari, Erwanto Y, Che-Man YB. Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Sci 2011;88(1):91-95.

Nagano I, Wu Z, Takahashi Y. Functional genes and proteins of TrichKinella spp. Parasitol Res 2009;104(2):197-207.

Bruschi F, Chiumiento L. Immunomodulation in trichinellosis: does Trichinella really scape the host immune system?. Endocr Metab Immune Disord Drug Targets 2012;12(1):4-15.

Morelle W, Haslam SM, Morris HR, Dell A. Characterization of the N-linked glycans of adult Trichinella spiralis. Mol Biochem Parasitol 2000;109(2):171-177.

Boonmars T, Wu Z, Nagano I, Takahashi Y. Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection. Parasitol Res 2005;97(1):13-20.

Ramírez-Melgar C, Gómez-Priego A, de-la-Rosa JL. Application of Giemsa stain for easy detection of Trichinella spiralis muscle larvae. Korean J Parasitol 2007;45(1):65-68.

Kotula AW, Murrell KD, Acosta-Stein L, Lamb L, Douglass L. Destruction of Trichinella spiralis during cooking. J Food Sci 1983;48(3):765-768.

Gamble HR, Bessonov AS, Cuperlovic K, Gajadhar AA, van-Knapen F, Noeckler K., et al. International Commission on Trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption. Vet Parasitol 2000;93(3-4):393-408.

Potter ME, Kruse MB, Matthews MA, Hill RO, Martin RJ. A sausage-associated outbreak of trichinosis in Illinois. Am J Public Health 1976;66(12):1194-1196.

Martínez-Marañón R. ¿Está aumentando la triquinosis en México? ¿Podría esto ser una consecuencia inesperada de nuestro "desarrollo"? Salud Publica Mex 1985;27(1):40-51.

Zamora-Chávez A, de-la-O-Cavazos ME, Bernal-Redondo RM, Berrones-Espericueta D, Vázquez-Antona C. Triquinosis aguda en niños. Brote epidémico intrafamiliar en la ciudad de México. Bol Med Hosp Infant Mex 1990;47(6):395-400.

Krivokapich SJ, Graciana MG, Gonzalez-Prous CL, Degese MF, Arbusti PA, Ayesa GE, et al. Detection of Trichinella britovi in pork sausage suspected to be implicated in a human outbreak in Mendoza, Argentina. Parasitol Int 2019;71:53-55.

Saldaña, M. Aumentan mexicanos su consumo de embutidos. EL UNIVERSAL 2018; (16/02/2018).https://www.eluniversal.com.mx/cartera/aumentan-mexicanos-su-consumo-de-embutidos. Consultado 12 Dic, 2020.

Publicado

11.02.2022

Cómo citar

de la Rosa Arana, J. L., Ponce Noguez, J. B., Gallardo Velázquez, T., Reyes Rodríguez, N. E., Zepeda Velázquez, A. P., López Lugo, A. B., … Gómez De Anda, F. R. (2022). Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig. Revista Mexicana De Ciencias Pecuarias, 12(4), 1133–1150. https://doi.org/10.22319/rmcp.v12i4.5897
Metrics
Vistas/Descargas
  • Resumen
    871
  • PDF
    342
  • PDF
    331

Métrica

Artículos similares

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 > >> 

También puede Iniciar una búsqueda de similitud avanzada para este artículo.

Artículos más leídos del mismo autor/a