Detección del virus de la lengua azul en ovinos por RT- PCR en tiempo real en diferentes sistemas de producción en San Martín, Perú
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https://doi.org/10.22319/rmcp.v13i3.5873Palabras clave:
ARN, Gen, Diagnóstico molecular, Rumiantes, AmazoniaResumen
El presente estudio tuvo como objetivo determinar la prevalencia del Virus de la Lengua Azul (VLA) en ovinos, por la técnica reacción en cadena de la polimerasa con Transcripción Reversa (RT- PCR) en tiempo real. Se evaluaron 366 ovinos procedentes de las diez provincias de la región del Perú. La metodología empleada fue la toma de muestras sanguínea de la vena yugular del ovino, seguidamente se realizó el proceso de extracción y purificación de ARN con el kit QIAmp®, luego la transcripción reversa para obtener el ADNc, y finalmente realizar la RT- PCR en tiempo real, para lo cual se utilizó el kit SuperScript III platinium One- step qRT–PCR, siendo los iniciadores y sondas dirigidos al segmento 10 del gen NS3 del VLA. Los resultados de la prueba de RT-PCR en tiempo real revelaron dos ovinos positivos con valor de ciclo umbral (Ct) de 35.21 y 35.57, siendo la prevalencia de 0.54 % de ovinos positivos a VLA en sistema de crianza extensiva, con las condiciones del ambiente que favorecen el desarrollo del vector Culicoide. Se concluye que mediante la técnica RT-PCR en tiempo real, se confirma la presencia del VLA en esta región del Perú, lo que hace necesario futuros estudios para determinar la detección de otros serotipos potenciales de VLA en la Amazonia peruana, con la finalidad de mejorar las estrategias de control de la enfermedad.
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