Thymol and carvacrol determination in a swine feed organic matrix using Headspace SPME-GC-MS

Autores/as

  • Fernando Jonathan Lona-Ramírez Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México. http://orcid.org/0000-0001-7924-2309
  • Nancy Lizeth Hernández-López Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.
  • Guillermo González-Alatorre Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.
  • Teresa del Carmen Flores-Flores Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.
  • Rosalba Patiño-Herrera Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México. http://orcid.org/0000-0002-0057-9310
  • José Francisco Louvier-Hernández Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México. http://orcid.org/0000-0002-1340-9863

DOI:

https://doi.org/10.22319/rmcp.v14i1.5394

Palabras clave:

Essential oil, Thymol, Carvacrol, Oregano, Origanum, HS-SPME-GC-MS

Resumen

In recent years, oregano essential oil has been used as an animal food additive due to its antifungal and antibacterial properties as well as s synthetic antibiotic substitute. It is desirable to develop fast and effective thymol and carvacrol quantification method in a swine feed organic matrix. In this work, a performance comparison between the Soxhlet solvent extraction technique using petroleum ether and ethyl acetate and the head space-solid phase microextraction (HS-SPME) technique is made. A 24 design of experiments is performed for defining HS-SPME parameters: equilibrium temperature of 40 ºC, extraction temperature of 40 ºC, ionic strength of 0.57 M, and extraction time of 40 min. The HS-SPME method is more efficient for extracting thymol and carvacrol extraction from an organic matrix. Limits of detection and quantification values using Soxhlet extraction with ethyl acetate were 3.7 and 12.5 μL-1 for thymol and 1.4 and 4.7 μg L-1 for carvacrol, respectively; while LOD and LOQ for HS-SPME were 0.9 and 3.1 μg L-1 for thymol and 0.6 and 1.9 μg L-1 for carvacrol, respectively. The head space-solid phase microextraction method has the potential for quality control in the industry for active compounds present in oregano’s essential oil as an additive into an organic matrix.

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Biografía del autor/a

Fernando Jonathan Lona-Ramírez, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Ingeniería Ambiental 

Coordinador

Nancy Lizeth Hernández-López, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Departamento de Ingeniería Química

Guillermo González-Alatorre, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Departamento de Ingeniería Química 

Profesor Investigador

Teresa del Carmen Flores-Flores, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Departamento de Ingeniería Química 

Profesora Investigadora 

Rosalba Patiño-Herrera, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Departamento de Ingeniería Química 

Profesora Investigadora 

José Francisco Louvier-Hernández, Tecnológico Nacional de México en Celaya. Departamento de Ingeniería Química. Guanajuato, México.

Departamento de Ingeniería Química 

Profesor Investigador 

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Publicado

27.12.2022

Cómo citar

Lona-Ramírez, F. J., Hernández-López, N. L., González-Alatorre, G., Flores-Flores, T. del C., Patiño-Herrera, R., & Louvier-Hernández, J. F. (2022). Thymol and carvacrol determination in a swine feed organic matrix using Headspace SPME-GC-MS. Revista Mexicana De Ciencias Pecuarias, 14(1), 78–93. https://doi.org/10.22319/rmcp.v14i1.5394
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