Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces

Autores/as

  • Nydia Edith Reyes-Rodriguez Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.
  • Jeannette Barba-León Universidad de Guadalajara. Centro Universitario de Ciencias Biológicas y Agropecuarias, Departamento de Salud Pública. Zapopan, Jalisco, México.
  • Armando Navarro-Ocaña Universidad Nacional Autónoma de México. Facultad de Medicina, Departamento de Salud Pública. Ciudad Universitaria, México.
  • Vicente Vega-Sanchez Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.
  • Fabian Ricardo Gómez De Anda Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.
  • Juan Martin Talavera-Gonzalez Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México.
  • Martin Talavera-Rojas Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México. https://orcid.org/0000-0003-0908-985X

DOI:

https://doi.org/10.22319/rmcp.v11i4.5049

Palabras clave:

Cattle carcasses, Escherichia coli, Serotyping, stx2

Resumen

Shiga toxin E. coli (STEC) is an important pathogen responsible for foodborne illness, this have been related with epidemic outbreaks in the past, mainly because of consumption of bovine meat. The objective of this study was identify the serotypes and Stx2 subtypes and associate them with their possible epidemiology. There were analyzed a total of 65 isolates from the collection of the Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia of the Universidad Autónoma del Estado de México, from carcasses and feces of bovines at three different Municipal slaughterhouses. The identification of Stx2 gene by PCR at final point, sequencing and analyzed with the help of BLAST software. There were found O157:H7, O70:H16, O91:H10, O112ac:H2, O128ac:H26 serotypes, which have been reported to be present at infectious outbreaks previously by foodborne worldwide; 63.07% (41/65) of the Escherichia coli strains got amplified for Stx2 and after BLAS analysis it was confirmed its presence and a hypothetic protein. The presence of this serotypes in combination with different subtype’s, Stx2a, Stx2c, Stx2d, in carcasses and feces of bovine in must be considered as a potential risk for diseases an important problem of the public health.

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Biografía del autor/a

Nydia Edith Reyes-Rodriguez, Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.

Profesor tiempo completo

Jeannette Barba-León, Universidad de Guadalajara. Centro Universitario de Ciencias Biológicas y Agropecuarias, Departamento de Salud Pública. Zapopan, Jalisco, México.

Profesor Tiempo Completo

Armando Navarro-Ocaña, Universidad Nacional Autónoma de México. Facultad de Medicina, Departamento de Salud Pública. Ciudad Universitaria, México.

Profesor tiempo completo

Vicente Vega-Sanchez, Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.

Profesor tiempo completo

Fabian Ricardo Gómez De Anda, Universidad Autónoma del Estado de Hidalgo. Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia. Tulancingo de Bravo, Estado de Hidalgo, México.

Profesor tiempo completo

Juan Martin Talavera-Gonzalez, Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México.

Posgrado en Ciencias Agropecuarias y Recursos Naturales

Martin Talavera-Rojas, Universidad Autónoma del Estado de México. Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Estudios Avanzados en Salud Animal. Toluca, Estado de México, México.

Profesor tiempo completo

Citas

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Publicado

18.12.2020

Cómo citar

Reyes-Rodriguez, N. E., Barba-León, J., Navarro-Ocaña, A., Vega-Sanchez, V., Gómez De Anda, F. R., Talavera-Gonzalez, J. M., & Talavera-Rojas, M. (2020). Serotypes and Stx2 subtyping of Shiga toxin producing Escherichia coli isolates from cattle carcasses and feces. Revista Mexicana De Ciencias Pecuarias, 11(4), 1030–1044. https://doi.org/10.22319/rmcp.v11i4.5049
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