The effects of Pyrantel-Oxantel on the Dipylidium caninum tapeworm: An in vitro study

Autores/as

  • Jair Millán-Orozco Universidad Autónoma del Estado de Morelos, Facultad de Ciencias Agropecuarias. Av. Universidad No. 1001, Col. Chamilpa . 62209, Cuernavaca, Morelos, México. Universidad Autónoma Agraria Antonio Narro. Departamento de Ciencias Médico Veterinarias. Torreón, Coahuila, México. http://orcid.org/0000-0002-7313-5059
  • Jersson Millán-Orozco Universidad Autónoma del Estado de Morelos, Facultad de Ciencias Agropecuarias. Av. Universidad No. 1001, Col. Chamilpa . 62209, Cuernavaca, Morelos, México. Asociación de Médicos Veterinarios Zootecnistas Especialistas en Bovinos de la Comarca Lagunera, A.C. Gómez Palacio, Durango, México. http://orcid.org/0000-0002-4540-5808
  • Miguel Ángel Betancourt-Alonso Escuela de Medicina Veterinaria y Zootecnia en Pequeñas Especies, Federación Canófila Mexicana A.C. Ciudad de México, México.
  • América Ivette Barrera-Molina Universidad Autónoma del Estado de Morelos, Facultad de Nutrición. Cuernavaca, Morelos, México. http://orcid.org/0000-0003-4696-2654
  • María Soledad Valledor Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.
  • Virginia Méndez Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.
  • Alejandra Larrea Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.
  • Martín Sebastián Lima Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.
  • Javier Morán-Martínez Universidad Autónoma de Coahuila. Facultad de Medicina. Torreón, Coahuila, México. https://orcid.org/0000-0002-6514-238X
  • Nadia Denys Betancourt-Martínez Universidad Autónoma de Coahuila. Facultad de Medicina. Torreón, Coahuila, México. https://orcid.org/0000-0001-7288-8705
  • Liliana Aguilar-Marcelino Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Jiutepec, Morelos, México. https://orcid.org/0000-0002-8944-5430

DOI:

https://doi.org/10.22319/rmcp.v12i3.5014

Palabras clave:

Dipylidium caninum, Helminths, Zoonosis, Motility, Morphology, Cestocidal effect

Resumen

The present study aimed to evaluate, in vitro, the cestocidal effect of Pyrantel-Oxantel on the Dipylidium caninum tapeworm. Each intestine sample was obtained by means of a transversal incision of the abdominal area of each euthanized canine subject, individually dissected via  longitudinal incision, and examined for the presence of D. caninum. An optical microscope was used to identify and verify proglottid morphology and viability based on its macroscopic appearance. The cestocidal effects of Pyrantel-Oxantel (75 mg pyrantel pamoate; 75 mg oxantel pamoate) were assessed in adult tapeworms (treated group, n= 21; control group, n= 21) placed on Petri dishes and incubated at 37 °C. One-hour post-incubation, the D. caninum cestodes treated with Pyrantel-Oxantel presented a 28 % decrease (P=0.001) in motility, which rose to a 52 % (P=0.0001) decrease by the end of the second hour. The control group (P=0.0001) presented 55.7 % motility for at least the first six hours of incubation and 4.2 % (P=0.001) motility by the end of the study, while 0 % motility was observed in the treated group by the end of the study. Pyrantel-Oxantel had a lethal effect (P=0.0001) on adult D. caninum, with 100 % mortality observed 6 h after in vitro post-incubation, while the control group presented 55.7 % viability after the same time period. In addition, Pyrantel-Oxantel reduced (P=0.001) tegument thickness by 42.5 % (10.24 ± 0.21 µm), while this was 17.81 ± 0.33 µm for the control group. The results of this study indicate that Pyrantel-Oxantel has a therapeutic effect on the presence of D. caninum, inducing both a reduction of the tegument thickness and increased mortality.

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Biografía del autor/a

Jair Millán-Orozco, Universidad Autónoma del Estado de Morelos, Facultad de Ciencias Agropecuarias. Av. Universidad No. 1001, Col. Chamilpa . 62209, Cuernavaca, Morelos, México. Universidad Autónoma Agraria Antonio Narro. Departamento de Ciencias Médico Veterinarias. Torreón, Coahuila, México.

Laboratorio de Anatomía

Departamento de Ciencias Médico Veterinarias 

Profesor Investigador de Tiempo Completo 

Jersson Millán-Orozco, Universidad Autónoma del Estado de Morelos, Facultad de Ciencias Agropecuarias. Av. Universidad No. 1001, Col. Chamilpa . 62209, Cuernavaca, Morelos, México. Asociación de Médicos Veterinarios Zootecnistas Especialistas en Bovinos de la Comarca Lagunera, A.C. Gómez Palacio, Durango, México.

Asociación de Médicos Veterinarios Zootecnistas Especialistas en Bovinos de la Comarca Lagunera, A.C.

Miguel Ángel Betancourt-Alonso, Escuela de Medicina Veterinaria y Zootecnia en Pequeñas Especies, Federación Canófila Mexicana A.C. Ciudad de México, México.

Escuela de Medicina Veterinaria y Zootecnia en Pequeñas Especies

Federación Canófila Mexicana, A.C.

América Ivette Barrera-Molina, Universidad Autónoma del Estado de Morelos, Facultad de Nutrición. Cuernavaca, Morelos, México.

Secretaría de Investigación y Posgrado

Facultad de Nutrición

Cooordinadora de Posgrado

María Soledad Valledor, Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.

Departamento de Parasitología y Enfermedades Parasitarias

Facultad de Veterinaria

Profesor Investigador

Virginia Méndez, Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.

Área de Anatomía

Departamento de Morfología y Desarrollo

Profesor Investigador

Alejandra Larrea, Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.

Área de Anatomía

Departamento de Morfología y Desarrollo

Profesor Investigador

Martín Sebastián Lima, Universidad de la República. Facultad de Veterinaria. Montevideo, Uruguay.

Área de Anatomía

Departamento de Morfología y Desarrollo

Profesor Investigador

Javier Morán-Martínez, Universidad Autónoma de Coahuila. Facultad de Medicina. Torreón, Coahuila, México.

Departamento de Biología Celular y Ultraestructura

Centro de Investigación Biomédica

Facultad de Medicina

Universidad Autónoma de Coahuila

Nadia Denys Betancourt-Martínez, Universidad Autónoma de Coahuila. Facultad de Medicina. Torreón, Coahuila, México.

Departamento de Biología Celular y Ultraestructura

Centro de Investigación Biomédica

Facultad de Medicina

Universidad Autónoma de Coahuila

Liliana Aguilar-Marcelino, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Jiutepec, Morelos, México.

Área de Helmintología

Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad

Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias

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Publicado

15.12.2021

Cómo citar

Millán-Orozco, J., Millán-Orozco, J., Betancourt-Alonso, M. Ángel, Barrera-Molina, A. I., Valledor, M. S., Méndez, V., … Aguilar-Marcelino, L. (2021). The effects of Pyrantel-Oxantel on the Dipylidium caninum tapeworm: An in vitro study. Revista Mexicana De Ciencias Pecuarias, 12(3), 969–986. https://doi.org/10.22319/rmcp.v12i3.5014
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