In vitro ruminal degradation of carbohydrate fractions in tropical grasses fertilized with nitrogen
DOI:
https://doi.org/10.22319/rmcp.v11i1.4829Palabras clave:
Tropical grasses, Carbohydrate fractions, Digestion rates, gas production, CNCPSResumen
Our goal was to determine the digestion rates of carbohydrate fractions A (sugars, oligosaccharides and organic acids), B1 (starch and soluble fiber), NSC (non-structural carbohydrates) and B2 (available NDF) in four tropical grasses using the gas production technique. Samples of whole forage (WF), residue insoluble in 90% ethanol (EIR) and isolated NDF (ND) were fermented in vitro and gas production measured. Gas volumes determined from the following fractions, A = WF minus EIR; B1 = EIR - ND; NSC = WF - ND; and B2 = ND. Grasses were Andropogon gayanus, Urochloa brizantha, Cynodon plectostachyus, and Megathyrsus maximum each grown in Veracruz, Mexico on four plots (5×5 m), fertilized (relationship equivalent to 0 and 100 kg N/ha) and clipped 35 d after the N fertilization. A complete randomized block design with factorial arrangement 4×2 and two replicates per treatment was used. Factors were grass species and N fertilization. Data were fit using a single-pool exponential model with lag. The volume (mL gas/100 mg OM), rate (%/h) and lag (h) were: WF (22.8; 5.3; 2.1); A (3.2; 15.7; 0.5); B1 (1.5; 15.7; 0.2); and B2 (18.3; 6.6; 5.2). Andropogon and Urochloa had higher NSC content compared to Megathyrsus and Cynodon but lower gas yield per unit of NSC. Rates of digestion for the B2 fraction ranged from 4 to 8 %/h; and NSC digestion rate averaged 15.7 %/h. Nitrogen fertilization reduced carbohydrate pool sizes but did not affect rates of digestion. It is concluded that ruminally available energy from SC and NSC in tropical forages should be revised more extensively.Descargas
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Van Soest PJ. Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fiber and lignin. JAOAC. 1963:(46):829-835.
Van Soest PJ. Development of a comprehensive system of feed analyses and its application to forages. J Anim Sci 1967:(26):119-128.
Tilley JMA, and Terry RA. A two stage technique for the in vitro digestion of forage of forage crops. J Br Grassland Soc 1963:(18):104-111.
Sniffen CJ, O'Connor JD, Van Soest PJ, Fox DG, Russell JB. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci 1992;(70):3562-3577.
Fox DG, Tedeschi LO, Tylutki TP, Russell JB, Van Amburgh ME, Chase LE, Pell AN, and Overton TR. The Cornell Net Carbohydrate and Protein System model for evaluating herd nutrition and nutrient excretion. Anim Feed Sci Technol 2004:(112)(1-4):29-78.
Lanzas C, Sniffen CJ, Seo S, Tedeschi LO, and Fox DG. A revised CNCPS feed carbohydrate fractionation scheme for formulating rations for ruminants. Anim Feed Sci Technol 2007:(136):167–190.
Van Amburgh ME, Collao-Saenz EA, Higgs RJ, Ross DA, Recktenwald EB, Raffrenato E, et al. The Cornell Net Carbohydrate and Protein System: Updates to the model and evaluation of version 6.5. J Dairy Sci 2015;(98):6361-6380.
Doane PH, Pell AN, Schofield P. Ensiling effects on the ethanol fractionation of forages using gas production. J Anim Sci 1998;76(3):888-895.
Molina DO. Prediction in intake of lactating cows in the tropics and of energy value of organic acids [doctoral thesis]. Ithaca, New York, USA: Cornell University; 2002.
Menke KHL, Raab A, Salewski H, Steingass D, Fritz Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci (Camb) 1979:(93):217-222.
Pell AN, Schofield P. Computerized monitoring of gas production to measure forage digestion in vitro. J Dairy Sci 1993;(76):1063-1073.
Schofield P, Pell AN. Measurement and kinetic analysis of the neutral detergent-soluble carbohydrate fraction of legumes and grasses. J Anim Sci 1995:(73):3455-3463.
Nutrient Requirements of Beef Cattle. 2016. National Academies of Sciences, Engineering, and Medicine. Eighth revised ed. Washington, DC: The National Academic Press.
Large Ruminant Nutrition System (LRNS) v1.033 http://nutritionmodels.com/lrns.html
Juarez-Lagunes FI, Fox DG, Blake RW, Pell AN. Evaluation of tropical grasses for milk production by dual-purpose cows in tropical Mexico. J Dairy Sci 1999;(82):2136-2145.
Ki Kwang Seok, Su Bum Park, Dong Hyun Lim, Seongwon Seo. Evaluation of the nutritional value of locally produced forage in Korea using chemical analysis and in vitro ruminal fermentation. Asian-Australas J Anim Sci 2017:(30):(3):355-362.
Carvalho P, Da Silva CL, Nalcino FP, Scatolin OI, Dias LPR, Moreno FM. In vitro kinetic parameters of marandu palisadegrass associated with nonfiber carbohydrates. Semina: Ciências Agrárias, Londrina 2016:(37):(5):3253-3264.
Ribeiro RCO, Villela SDJ, Valadares Filho SC, Santos SA, Ribeiro KG, Detmann E, Zanetti D, Martins PGMA. Effects of roughage sources produced in a tropical environment on forage intake, and ruminal and microbial parameters. J Anim Sci 2015:(93):2363–2374.
Tiwari UP, Turano B, Jha R. Nutritional characteristics and in vitro digestibility by near-infrared spectroscopy of local and hybrid napiergrass varieties grown in rain-fed and irrigated conditions. Anim Prod Sci 2014:(54):1775–1778.
AOAC Official methods of analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists. 1990.
Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991:(74):3583-3597.
Goering HK, Van Soest PJ. Forage fiber analysis (apparatus, reagents, procedures, and some applications). Washington, DC, USA: Agric. Handbook No. 379. ARS-USDA; 1970.
Hall MB, Lewis BA, Van Soest PJ, Chase LE. A simple method for estimation of neutral-detergent soluble fiber. J Sci Food Agric 1997:(74):441-449.
Schofield P, Pell AN. Validity of using accumulated gas pressure readings to measure forage digestion in vitro: a comparison involving three forages. J Dairy Sci 1995:(78):2230-2238.
Mertens DR, Loften JR. The effect of starch on forage fiber digestion kinetics in vitro. J Dairy Sci 1980:(63):1437-1446.
Stefanon B, Pell AN, Schofield P. Effect of maturity on digestion kinetics of water-soluble and water-insoluble fractions of alfalfa and brome hay. J Anim Sci 1996:(74):1104-1115.
Cone JW, Van Gelder AH, Visscher GJW, and Oudshoorn L. Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Anim Feed Sci Technol 1996:(61):113-128.
Minitab Inc. Minitab Reference Manual, Release 10, PC Version. Minitab Stat. Software. State College, Pensilvania, USA. 1994.
Arzate-Vázquez GL, Castrejón-Pineda FA, Rosiles-Martínez R, Carrillo-Pita S, Ángeles-Campos S, Vargas-Bello-Pérez E. Effect of genus and growth stage on the chemical and mineral composition of tropical grasses used to feed dairy cows. Cien Inv Agr 2016;43(3):476-485.
Lim F, Wong CC. Nutritive values and correlation equations on some improved tropical forages. MARDI Res J 1997;(202):173-180.
Ramos-Santana R, McDowell LR. In vitro digestibility, crude protein content, and mineral concentrations of Cynodon, Brachiaria and Digitaria accessions in a humid tropical region of Puerto Rico. Commun Soil Sci Plant Annal 1996:(27):2687-2697.
Grant RJ, Perez CB Jr, Van Soest PJ, McDowell RE. Composition and in vitro true digestion of some Philippine feedstuffs. Philippine J Anim Sci 1973:(10):63-76.
Arroyo-Aguilú JA, Tessema S, McDowell RE, Van Soest PJ, Ramírez A, Randel PF. Chemical composition and in vitro digestibility of five heavily fertilized tropical grasses in Puerto Rico. J Agric Puerto Rico 1975:(59):186-198.
Poczynek Mailson, Mikael Neumann, Egon Henrique Horst, Bruno José Venancio, Danúbia Nogueira Figueira, Milaine Poczynek, Sandra Galbeiro. Mass and nutritional quality of upper and lower strata of tropical forages. Semina: Ciências Agrárias, Londrina 2016:(37):(4):Supl 1:2725-2736.
Espinoza-Guerra I, Pérez-Oñate C, Montenegro-Vivas L, Sánchez-Liaño A, García-Martínez A, Martínez-Marín AL. Composition and in vitro rumen degradation kinetics of saboya grass (Megathyrsus maximus) silage with inclusion of passion fruit rind (Passiflora edulis Sims.). Rev Cient FCV-LUZ / 2016:(26):(6):402–407.
Lima Karla Rodrigues De, Carlos Augusto Brandão De Carvalho, Flavio Henrique Vidal Azevedo, Pedro Antonio Muninz Malafaia. Sward structure and nutritive value of Urochloa ruziziensis under nitrogen and potassium fertilization. Rev Caatinga Mossoró 2017:(30):(1):220–229.
Van Soest PJ. Nutritional ecology of the ruminant. Ithaca, New York, USA. Cornell University Press. 1994.
Weiss WP, Conrad HR, St. Pierre NR. A theoretically-based model for predicting total digestible nutrient values of forages and concentrates. Anim Feed Sci Technol 1992;(39):95-110.
Fukushima RS, Bacha CB, Fuzeto AP, Port ACR, Herling VR, Velasquez AV. Utilization of equations to predict carbohydrate fractions in some tropical grasses. Anim Feed Sci Technol 2015:(208):12-22.
Bezabih M, Pellikaan WF, Tolera A, Khan NA, Hendriks WH. Chemical composition and in vitro total gas and methane production of forage species from the Mid Rift Valley grasslands of Ethiopia. Grass Forage Sci 2013:(69):4.
Ellis RP. Tannin-like substances in grass leaves. Mem Bot Survey S Africa 1990:(59):3-78.
Oliveira Ednaldo M. De, Jair Da C. Oliveira Filho, Rubens A. De Oliveira, Reginaldo M. De Oliveira, Paulo R. Cecon. Determination of Xaraés grass quality submitted to irrigation water levels and nitrogen and potassium doses. J Brazilian Assoc Agr Engineering 2017:(37):(1):64-74.
Campos FP, Nicacio DRO, Sarmento P, Cruz MCP, Santos TM, Faria AFG, Ferreira ME, Conceicao MRG, Lima CG. Chemical composition and in vitro ruminal digestibility of hand-plucked samples of Xaraes Palisade grass fertilized with incremental levels of nitrogen. Anim Feed Sci Technol 2016:(215):1-12.
Campos FP, Sarmento P, Nussio LG, Lugão SMB, Lima CG. Fiber monosaccharides and digestibility of Milenio grass under N fertilization. Anim Feed Sci Technol 2013:(183):17-21.
Mertens DR. Application of theoretical mathematical models to cell wall digestion and forage intake in ruminants [doctoral thesis]. Ithaca, New York, USA: Cornell University; 1973.
Raffrenato E, Erasmus LJ. Variability of indigestible NDF in C3 and C4 forages and implications on the resulting feed energy values and potential microbial protein synthesis in dairy cattle. South African J Anim Sci 2013;43(5):(Suppl 1).
Huhtanen P, Seppala A, Ots M, Ahvenjarvi S, Rinne M. In vitro gas production profiles to estimate extent and effective first-order rate of neutral detergent fiber digestion in the rumen. J Anim Sci 2008:(86):651–659.
Detmann Edenio, José Fernando Coelho da Silva, Hernán Maldonado Vásquez, Lara Toledo Henriques, Ismail Ramalho Haddade. Kinetic parameters of carbohydrates ruminal degradation of four tropical grasses in different cutting ages and nitrogen fertilizer levels. R Bras Zootec 2009;38(1):149-158.
Wilson JR. Cell wall characteristics in relation to forage digestion by ruminants. J Agric Sci (Camb) 1994:(122):173-182.
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