Immunolocalization of VirB11 protein in the Anaplasma marginale outer membrane and its reaction with bovine immune sera

Autores/as

  • América Ivette Barrera Molina Universidad Nacional Autónoma de México. UNAM-FMVZ. Av. Universidad 3000, Edificio A, Delegación Coyoacán, Col. Ciudad Universitaria, 04510 Ciudad de México, México. b INIFAP-Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria. Tel: 52 800 088 2222 ext.80409 +52 777 3192850 ext. 125. CIVAC, Carr. Cuernavaca - Cuautla No 8534, Col. Progreso, 62550, Jiutepec, Mor. México.
  • Raquel Cossío Bayúgar INIFAP-Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria. Tel: 52 800 088 2222 ext.80409 +52 777 3192850 ext. 125. CIVAC, Carr. Cuernavaca - Cuautla No 8534, Col. Progreso, 62550, Jiutepec, Mor. México.
  • José Ángel Gutiérrez-Pabello Universidad Nacional Autónoma de México. UNAM-FMVZ. Av. Universidad 3000, Edificio A, Delegación Coyoacán, Col. Ciudad Universitaria, 04510 Ciudad de México, México.
  • Ángel Tolentino Tello López Instituto Nacional de Salud Pública (INSP). Cuernavaca, Mor. México.
  • Jesús Francisco Preciado de Torre INIFAP-Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria. Tel: 52 800 088 2222 ext.80409 +52 777 3192850 ext. 125. CIVAC, Carr. Cuernavaca - Cuautla No 8534, Col. Progreso, 62550, Jiutepec, Mor. México.
  • Sergio D. Rodríguez Camarillo CENID Parasitología INIFAP http://orcid.org/0000-0003-1316-5739

DOI:

https://doi.org/10.22319/rmcp.v9i4.4508

Palabras clave:

Anaplasma marginale, Type 4 secretion system, Multiple antigenic peptide, MAP, VirB11.

Resumen

Type IV secretion system is composed by a group of proteins associated with substrate transfer between bacteria and eukaryotic organisms. VirB11 is a protein of this system and, in A. marginale it induces partial protection in cattle immunized with external membrane fragments of the rickettsia. The objectives of this study were: to determine the conservation rate of VirB11 among different Mexican isolates and to corroborate its cellular localization on the outer membrane of A. marginale. Nucleotide and amino acid sequence alignment of virB11 from nine Mexican isolates showed 100 % identity. Bovine sera from both naturally and experimentally infected cattle reacted against a multiple antigenic peptide (MAP‑VirB11) designed based on a B-type epitope present in VirB11. In addition, MAP‑VirB11‑rabbit immune sera reacted with native VirB11 by ELISA and western blot in rickettsial extracts and on the surface of bovine infected erythrocytes by indirect immunofluorescence and the localization of VirB11 protein was determined on the outer surface of A. marginale initial bodies.

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Biografía del autor/a

América Ivette Barrera Molina, Universidad Nacional Autónoma de México. UNAM-FMVZ. Av. Universidad 3000, Edificio A, Delegación Coyoacán, Col. Ciudad Universitaria, 04510 Ciudad de México, México. b INIFAP-Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria. Tel: 52 800 088 2222 ext.80409 +52 777 3192850 ext. 125. CIVAC, Carr. Cuernavaca - Cuautla No 8534, Col. Progreso, 62550, Jiutepec, Mor. México.

Candidato a doctorado, La publicación es requisito para titulación

Raquel Cossío Bayúgar, INIFAP-Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria. Tel: 52 800 088 2222 ext.80409 +52 777 3192850 ext. 125. CIVAC, Carr. Cuernavaca - Cuautla No 8534, Col. Progreso, 62550, Jiutepec, Mor. México.

Investigadora Titular C.

Sergio D. Rodríguez Camarillo, CENID Parasitología INIFAP

Coordinador, Unidad de Anaplasmosis

Citas

Kocan KML, de la Fuente J, Guglielmone AA, Meléndez RD. Antigens and alternatives for control of Anaplasma marginale infection in cattle. Clin Microbiol Rev 2003;16(4):698-712.

Rodríguez-Vivas RI, Grisi L, Pérez de León AA, Silva Villela H, de Jesús Torres-Acosta JF, Fragoso Sánchez H, et al. Potential economic impact assessment for cattle parasites in Mexico. Rev Mex Cienc Pecu 2017;8(1):61-74.

Rodríguez CSD, García OMA, Rojas REE, Cantó AGJ, Preciado TJF, Rosario CR, et al., Anaplasma marginale Yucatan (Mexico) Strain. Assessment of low virulence and potential use as a live vaccine. Ann N Y Acad Sci 2008;1149:98–102.

Kocan KM, de la Fuente J, Blouin, EF, Coetzee JF, Ewing SA, The natural history of Anaplasma marginale. Vet Parasitol 2010;167:95–107.

Ueti MW, Reagan JO, Knowles DP, Scoles GA, Shkap V, Palmer GH. Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale. Infect Immun 2007;75:2959–2964.

Palmer GH, Knowles DP, Rodriguez JL, Gnad DP, Hollis LC, Marston T, et al. Stochastic transmission of multiple genotypically distinct Anaplasma marginale strains in a herd with high prevalence of Anaplasma infection. J Clin Microbiol 2004;42(11):5381–5384.

Palmer GH, McGuire TC. Immune serum against Anaplasma marginale initial bodies neutralizes infectivity for cattle. J Immunol 1984;133:1010-1015.

De la Fuente J, Van Den Bussche RA, Prado TM, Kocan KM. Anaplasma marginale msp1α genotypes evolved under positive selection pressure but are not markers for geographic isolates. J Clin Microbiol 2003;41:1609-1616.

Brayton KA, Kappmeyer LS, Herndon DR, Dark MJ, Tibbals DL, Palmer GH, et al. Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins. Proc Natl Acad Sci USA 2005;102:844–849.

Castañeda-Ortiz EJ, Ueti MW, Camacho-Nuez M, Mosqueda JJ, Mousel MR, Johnson WC, et al. Association of Anaplasma marginale strain superinfection with infection prevalence within tropical regions. PLoS One 2015;10(5):e0129415; Correction: PLoS One. 2015;10(5):e0129415.

Sutten EL, Norimine J, Beare PA, Heinzen RA, Lopez JE, Morse K, et al. Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine. Infect Immun 2010;78:1314–1325.

González-Pedrajo B, Dreyfus G, Sistemas de secreción de proteínas en las bacterias Gram negativas: Biogénesis flagelar y translocación de factores de virulencia. Mensaje Bioquim 2003;27:45–63.

Sutten EL, Norimine J, Beare PA, Heinzen RA, Lopez JE, Morse K, et al. Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine. Infect Immun 2010;78:1314–1325.

Apostolopoulos V. Vaccine delivery methods into the future. Vaccines (Basel) 2016;4(2):9. DOI:https://doi.org/10.3390/vaccines 402009.

Jiménez Ocampo R, Rodríguez Camarillo SD, Rosario R, Orozco Vega LE, de la Fuente J. Anaplasma marginale: análisis de las secuencias del fragmento variable del gen msp1 α y del gen msp4 de cuatro nuevas cepas mexicanas. Téc Pecu Méx 2008;46(1):69–78.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann Biochem 1976;72:248-254.

Eddy SR. Multiple alignment using hidden Markov models. Proc Int Conf Intell Syst Mol Biol 1995;3:114-120.

Möller S, Croning MD, Apweiler R. Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 2001;17(7):646-653.

Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, et al. Protein Identification and analysis tools on the ExPASy Server; In: John M. Walker editor. The proteomics protocols handbook. USA: Humana Press; 2005:571-607

Saha S, Raghava GPS. BcePred: Prediction of continuous B-cell epitopes in antigenic sequences using physico-chemical properties. In: Nicosia G, et al. editors. ICARIS, LNCS 3239. Germany: Springer; 2004:197-204.

Saha S, Raghava GP. Prediction of continuous B-cell epitopes in an antigen using recurrent neural network. Proteins 2006;65(1);40–48.

Oliveira B, Araújo R, Melo V, Madruga C, Soares C, Kessler R. et al. Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale; Mem Inst Oswaldo Cruz, 2005;100(7):765-769.

Brayton KA, Kappmeyer LS, Herdon DR, Dark MJ, Tibbals DL, Palmer GH, et al. Complete genome sequencing or Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins. Proc Natl Acad Sci 2005;102(3):844-849.

Sutten EL, Norimine J, Beare PA, Heinzen RA, Lopez JE, Morse K, et al. Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine. Infect Immun 2010;78:1314–1325.

Gillespie JJ, Phan IQH, Driscoll TP, Guillotte ML, Lehman SS, Rennoll-Bankert KE, et al. The Rickettsia type IV secretion system: Unrealized complexity mired by gene family expansion. Pathog Dis 2016;74(6):ftw058.

Niu H, Rikihisa Y, Yamaguchi M, Ohashi N. Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway. Cell Microbiol 2006;8(3):523–534.

Publicado

27.09.2018

Cómo citar

Barrera Molina, A. I., Cossío Bayúgar, R., Gutiérrez-Pabello, J. Ángel, Tello López, Ángel T., Preciado de Torre, J. F., & Rodríguez Camarillo, S. D. (2018). Immunolocalization of VirB11 protein in the Anaplasma marginale outer membrane and its reaction with bovine immune sera. Revista Mexicana De Ciencias Pecuarias, 9(4), 769–791. https://doi.org/10.22319/rmcp.v9i4.4508
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