In vitro ruminal degradation of neutral detergent fiber insoluble protein from tropical pastures fertilized with nitrogen


  • Francisco Indalecio Juárez Lagunes Facultad de medicina Veterinaria y Zootecnia, Universidad Veracruzana
  • Alice N. Pell Cornell University. Animal Science Department. Ithaca, NY. USA.
  • Robert W. Blake Cornell University. Animal Science Department. Ithaca, NY. USA.
  • Maribel Montero Lagunes INIFAP. Campo Experimental La Posta. Medellín, Ver. México.
  • Juan Manuel Pinos Rodríguez Universidad Veracruzana. Facultad de Medicina Veterinaria y Zootecnia. CP. 91710. Veracruz, Ver. México.


Palabras clave:

Tropical grasses, Degradation kinetics, Protein fractions, NDF, N fertilization.


The objective was to determine in vitro the NDF insoluble protein (NDIP) extension and degradation rate of four tropical grasses by the potential effect of N fertilization. The grasses (Andropogon gayanus, Brachiaria brizantha, Cynodon plectostachyus and Megathyrsus maximus) that grow in Mexico were used. Each grass was grown in four plots (5×5 m), fertilized (relationship equivalent to 0 and 100 kg N/ha) and clipped 35 d after the N fertilization. A complete randomized block design with factorial arrangement 4×2, and two replicates per treatment was used, where the factors were grass species and N fertilization. Non-protein nitrogen (NPN), buffer insoluble protein (IP), NDIP and acid detergent insoluble protein (ADIP) were performed. Freeze-dried samples were incubated at 0, 1.5, 3, 6, 9, 12, 24, 48 and 96 h. After fermentation, the CP content of the NDF residues was determined. An exponential equation was used to determine the rate of the NDIP disappearance. There was no detectable interaction between type of grass and fertilization level. The NDIP (as %CP) averaged 35 % with a range of 10 to 60 %. The NDIP variation was primarily due to species. The extent and rates of degradation of the NDIP were 70.6 % and 7.1 %/h respectively, with no N-fertilization effect. The NDIP was degraded faster (P≤0.05) than NDF (7.7 vs 5.0 %/h). These data show that the NDIP is ruminally degraded and that this fraction significantly contributes to the rumen nitrogen supply.


Los datos de descargas todavía no están disponibles.

Biografía del autor/a

Francisco Indalecio Juárez Lagunes, Facultad de medicina Veterinaria y Zootecnia, Universidad Veracruzana

Investigador Titular de Tiempo Completo. Universidad Veracruzana adscrito a la Facultad de Medicina Veterinaria y Zootecnia


Juarez-Lagunes FI, Fox DG, Blake RW, Pell AN. Evaluation of tropical grasses for milk production by dual-purpose cows in tropical Mexico. J Dairy Sci 1999;(82):2136-2145.

Singh S, Anele UY, Edmunds B, Südekum K-H. In vitro ruminal dry matter degradability, microbial efficiency, short chain fatty acids, carbohydrate and protein fractionation of tropical grass-multipurpose tree species diets. Liv Sci 2014;(160):45-51.

Van Amburgh ME, Collao-Saenz EA, Higgs RJ, Ross DA, Recktenwald EB, Raffrenato E, et al. The Cornell Net Carbohydrate and Protein System: Updates to the model and evaluation of version 6.5. J Dairy Sci 2015;(98):6361-6380.

Higgs RJ, Chase L.E, Ross DA, Van Amburgh ME. Updating the Cornell Net Carbohydrate and Protein System feed library and analyzing model sensitivity to feed inputs. J Dairy Sci 2015;(98):6340-6360.

Ogden RK, Coblentz WK, Coffey KP, Turner JE, Scarbrough DA, Jennings JA, Richardson MD. Ruminal in situ disappearance kinetics of nitrogen and neutral detergent insoluble nitrogen from common crabgrass forages sampled on seven dates in northern Arkansas. J Anim Sci 2006;(84):669-677.

Bowen NK, Poppi DP, McLennan SR. Ruminal protein degradability of a range of tropical pastures. Aust J Exp Agric 2008;(48):806-810.

Sniffen CJ, O'Connor JD, Van Soest PJ, Fox DG, Russell JB. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. J Anim Sci 1992;(70):3562-3577.

Campos, PR de SS, da Silva JFC, Vásquez HM, Vittori A, Almeida e Silva M. Fractions of carbohydrates and of nitrogenous compounds of tropical grasses at different cutting ages. R Bras Zootec 2010;39(7):1538-1547.

Singh S, Kushwaha BP, Nag SK, Mishra AK, Singh A, Anele UY. In vitro ruminal fermentation, protein and carbohydrate fractionation, methane production and prediction of twelve commonly used Indian green forages. Anim Feed Sci Technol 2012;(178):2-11.

Johnson CR, Reiling BA, Mislevy P, Hall MB. Effects of nitrogen fertilization and harvest date on yield, digestibility, fiber, and protein fractions of tropical grasses. J Anim Sci 2001;(79):2439-2448.

AOAC Official methods of analysis. 15th ed. Arlington, VA, USA: Association of Official Analytical Chemists. 1990.

Licitra G, Hernandez TM, Van Soest PJ. Standardization of procedures for nitrogen fractionation of ruminant feeds. Anim Feed Sci Technol 1996;(57):347-358.

Pichard G, Van Soest PJ. Protein solubility of ruminant feeds. Proc Cornell Nutrition Conf Feed Manufacturers. Ithaca, New York, USA, 1977:91-98.

Van Soest PJ. Nutritional ecology of the ruminant. Ithaca, New York, USA: Cornell University Press; 1994.

Goering HK, Van Soest PJ. Forage fiber analysis (apparatus, reagents, procedures, and some applications). Washington, DC, USA: Agric. Handbook No. 379. ARS-USDA; 1970.

Pell AN, Schofield P. Computerized monitoring of gas production to measure forage digestion in vitro. J Dairy Sci 1993;(76):1063-1073.

Kohn RA. In vitro methods to determine protein degradation of feeds for ruminants [doctoral thesis]. East Lansing, Michigan, USA: Michigan State University; 1993.

Mertens DR, Loften JR. The effect of starch on forage fiber digestion kinetics in vitro. J Dairy Sci 1980;(63):1437-1446.

Van Soest PJ, Mason VC. The influence of the Maillard reaction upon the nutritive value of fibrous feeds. Anim Feed Sci Technol 1991;(32):45-53.

Pal K, Patra AK, Sahoo A. Evaluation of feeds from tropical origin for in vitro methane production potential and rumen fermentation in vitro. Span J Agric Res 2015;13(3):e0608.

Salisbury FB, Ross CW. Plant physiology. Belmont, California, USA. Wadsworth Publishing Co.; 1992.

Brett C, Waldron K. Physiology and biochemistry of plant cell walls. London, UK: Chapman and Hall; 1996.

Valdes C, Andres S, Giraldez FJ, Garcia R, Calleja A. Potential use of visible and near infrared reflectance spectroscopy for the estimation of nitrogen fractions in forages harvested from permanent meadows. J Sci Food Agric 2006;(86):308-314.

Messman MA, Weiss WP, Erickson DO. Effects of nitrogen fertilization and maturity of bromegrass on nitrogen and amino acid utilization by cows. J Anim Sci 1992;(70):566-575.

Lamport DTA, Kieliszewski MJ, Chen Y, Cannon MC. Role of the extensin superfamily in primary cell wall architecture. Plant Physiol 2011;(156):11-19.

Kishor PBK, Hima Kumari P, Sunita MSL, Sreenivasulu N. Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny. Frontiers Plant Sci 2015;6:1-17. doi: 10.3389/fpls.2015.00544.

Anderson SJ, Klopfenstein TJ, Wilkerson VA. Escape protein supplementation of yearling steers grazing smooth brome pastures. J Anim Sci 1988;(66):237-242.

Castagnara DD, Mesquita EE, Neres MA, Oliveira PSR, Deminicis BB, Bamberg R. Nutritional value and structural characteristics of tropical grasses under nitrogen fertilization. Arch Zootec 2011;60(232):931-942.

Boschma SP, Murphy SR, Harden S. Herbage production and persistence of two tropical perennial grasses and forage sorghum under different nitrogen fertilization and defoliation regimes in a summer-dominant rainfall environment, Australia. Grass Forage Sci 2015;70(3):381-393.

Hoekstra NJ, Struik PC, Lantinga EA, Van Amburgh ME, Schulte RPO. Can herbage nitrogen fractionation in Lolium perenne be improved by herbage management? NJAS Wagen J Life Sci 2008;55(2):167-180.

Mertens DR. Application of theoretical mathematical models to cell wall digestion and forage intake in ruminants [doctoral thesis]. Ithaca, New York, USA: Cornell University; 1973.

Ogden RK, Coblentz WK, Coffey KP, Turner JE, Scarbrough DA, Jennings JA, Richardson MD. Ruminal in situ disappearance kinetics of dry matter and fiber in growing steers for common crabgrass forages sampled on seven dates in northern Arkansas. J Anim Sci 2005;(83):1142-1152.

LeRudulier D, Strom AR, Dandekar AM, Smith LT, Valentaine RC. Molecular biology of osmoregulation. Science 1984;(224):1064-1068.

Weiss WP, Conrad HR, St. Pierre NR. A theoretically-based model for predicting total digestible nutrient values of forages and concentrates. Anim Feed Sci Technol 1992;(39):95-110.



Cómo citar

Juárez Lagunes, F. I., N. Pell, A., Blake, R. W., Montero Lagunes, M., & Pinos Rodríguez, J. M. (2018). In vitro ruminal degradation of neutral detergent fiber insoluble protein from tropical pastures fertilized with nitrogen. Revista Mexicana De Ciencias Pecuarias, 9(3), 588–600.
  • Resumen
  • PDF
  • XML



Notas de investigación


Artículos similares

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 > >> 

También puede Iniciar una búsqueda de similitud avanzada para este artículo.

Artículos más leídos del mismo autor/a