Kisspeptina en becerras prepúberes: I. Influencia de la edad en la respuesta de LH, FSH y GH a kisspeptina-10 y su asociación con IGF-I, leptina y estradiol

Mónica Alamilla Rodríguez, René Carlos Calderón Robles, Jorge Víctor Rosete Fernández, Karla Rodríguez Hernández, Héctor Raymundo Vera Ávila, Jesús Alejandro Arreguín Arévalo, Terry M. Nett, Carlos Guillermo Gutiérrez Aguilar, Everardo González Padilla, Margarita Gómez-Chavarín, Alejandro Villa Godoy


El objetivo fue evaluar la respuesta de LH, FSH y GH a kisspeptina-10 (KISS) en becerras prepúberes de distintas edades, y su asociación con IGF-I, E2 y leptina circulantes. A 21 becerras de 4, 7 y 11 meses de edad se les aplicó una inyección i.v. de 5 µg de KISS/kg de peso. En suero obtenido de la yugular (cada 15 min, 4 h pre hasta 4 h post-KISS) se cuantificó LH, FSH y GH. En nueve muestras (tres pre y seis post-KISS) se determinó IGF-I, leptina y estradiol (E2). En todas las becerras aumentó la LH post-KISS, con un valor máximo (6.1±1.3a, 7.2±1.3a, 11.6+1.3b ng/ml) y duración de respuesta (64.3±20a, 92±20b, 132.9+20c min) que aumentó (P<0.05) con la edad. Para FSH, 71.4, 100 y 100 % de las becerras de 4, 7 y 11 meses respondieron a KISS-10; situación que para GH fue 71.4, 85.7 y 100 %, respectivamente. La magnitud de la respuesta de FSH y GH a KISS fue similar entre edades. Las becerras de 11 meses tuvieron las máximas concentraciones de IGF-I y E2 y las menores de leptina. Se concluye que KISS-10 evoca un aumento de LH en becerras de 4 a 11 meses, cuya magnitud de respuesta se asocia con la edad, con el incremento en sangre periférica de IGF-I y E2 y con la disminución de leptina sérica; en contraste, la respuesta de GH y FSH a KISS-10 no es afectada por la edad ni por las variaciones en IGF-I, E2 y leptina.

Palabras clave

Kisspeptina; Becerras prepúberes; LH; FSH; GH.

Texto completo:



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