Kisspeptina en becerras prepúberes: 2. Respuesta de LH, FSH y GH a distintas dosis de kisspeptina-10 y su asociación con IGF-I y leptina circulantes

Alejandro Villa Godoy, Rubén Santos Echeverría, Jorge Víctor Rosete Fernández, René Carlos Calderón Robles, Gerardo Perera Marín, Jesús Alejandro Arreguín Arevalo, Terry M. Nett

Resumen


En becerras prepúberes, la kisspeptina-10 (KISS-10) estimula la liberación de hormona luteinizante (LH) y hormona foliculoestimulante (FSH), pero su acción en la hormona del crecimiento (GH) es inconsistente. La hipótesis fue que en becerras prepúberes la magnitud de liberación de LH, FSH y GH en respuesta a KISS-10 es determinada por la dosis y las concentraciones endógenas de IGF-I y leptina. Se usaron 20 becerras prepúberes (100-232 kg de peso y 7-11 meses de edad), mismas que recibieron un bolo i.v. de 5 o 50 µg de  KISS-10/kg de peso. Ambas dosis de KISS-10 indujeron un incremento de LH y FSH, más no de GH. La dosis de 50 µg de KISS-10 indujo un área bajo la curva de LH y una duración del incremento de FSH mayores (P<0.05) que la dosis de 5 µg. IGF-I se relacionó negativamente con el aumento de LH en respuesta a la dosis de 50 µg (P<0.05), pero no estuvo asociado con la respuesta de LH a la dosis de 5 µg de KISS-10. La magnitud del incremento de FSH y las concentraciones de GH posteriores a KISS-10 no fueron alteradas por las concentraciones de IGF-I (P>0.05). De la misma forma, las concentraciones de leptina no determinaron (P>0.05) la magnitud del incremento de LH y FSH ni las concentraciones de GH pos tratamiento. En conclusión, la GH no respondió a ninguna dosis, pero la FSH y la LH fueron sensibles al aumento de dosis de KISS-10. Además, la magnitud de respuesta de LH a KISS-10 fue determinada por las concentraciones endógenas de IGF-I, pero no de leptina.


Palabras clave


Kisspeptina-10; Becerras prepúberes; LH; FSH; GH; IGF-I; Leptina.

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Referencias


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DOI: https://doi.org/10.22319/rmcp.v9i4.4192

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